Retrotransposon insertion into the maize waxy gene results in tissue-specific RNA processing

We previously reported that three alleles of the maize waxy (wx) gene were alternatively spliced as a result of the insertion of retrotransposons into intronic sequences. In addition, inefficient splicing of element sequences with the surrounding intron produced wild-type transcripts that presumably...

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Bibliographic Details
Published inThe Plant cell Vol. 9; no. 6; pp. 967 - 978
Main Authors Marillonnet, S. (Sainsbury Laboratory, Norwich, UK.), Wessler, S.R
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.06.1997
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ALLELES
ALTERNATIVE SPLICING
ARN MENSAJERO
ARN MESSAGER
Chromosome Mapping
Corn
ENDOSPERM
ENDOSPERMA
ENDOSPERME
ENZYMIC ACTIVITY
Exons
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENE EXPRESSION
GENES
GLICOSILTRANSFERASAS
GLYCOSYLTRANSFERASE
GLYCOSYLTRANSFERASES
HEXOSYLTRANSFERASES
Introns
MESSENGER RNA
MUTACION
MUTATION
Plant Proteins - genetics
POLEN
POLLEN
Pollen - genetics
Pollen - metabolism
Retroelements
Retrotransposons
RNA
RNA, Plant - metabolism
Seeds - genetics
Seeds - metabolism
Splicing
Starch Synthase - genetics
Transcription, Genetic
TRANSPOSITION
UX GENE
WXG ALLELE
ZEA MAYS
Zea mays - genetics
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Summary:We previously reported that three alleles of the maize waxy (wx) gene were alternatively spliced as a result of the insertion of retrotransposons into intronic sequences. In addition, inefficient splicing of element sequences with the surrounding intron produced wild-type transcripts that presumably were responsible for the observed residual gene expression in the endosperm. In this study, we report that one of these alleles, wxG, has a tissue-specific phenotype with 30-fold more WX enzymatic activity in pollen than in the endosperm. Quantification of wxG-encoded transcripts in pollen and the endosperm demonstrates that this difference can be accounted for by tissue-specific differences in RNA processing. Specifically, there is approximately 30-fold more correctly spliced RNA in pollen than in the endosperm. Based on an analogy to similar examples of tissue-specific alternative splicing in animal systems, we hypothesize that the tissue-specific phenotype of the wxG allele may reflect differences in the concentration of splicing factors in these tissues
Bibliography:1997051437
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ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.9.6.967