Transcriptome Analysis and Functional Characterization Reveal That Peclg Gene Contributes to the Virulence of Penicillium expansum on Apple Fruits

• Published in: Foods Vol. 12; no. 3; p. 479
• Main Authors: Zhou, Jiayu, Gong, Weifeng, Tu, Tingting, Zhang, Jiaqi, Xia, Xiaoshuang, Zhao, Luning, Zhou, Xinghua, Wang, Yun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.01.2023; MDPI
• Subjects: apple; Apples; Blue mold; Cell walls; Colonization; Computer applications; Concanavalin A; Disease; effector; Food science; Fruits; Fungi; Genes; Genetic engineering; Genomes; Germination; Health hazards; Host plants; Infections; Inoculation; Mold; Mutants; Mycotoxins; Pathogenesis; Pathogenicity; Pathogens; Patulin; Penicillium expansum; Proteins; Transcriptomes; Virulence; United States > US; China; Penicillium expansum; apple; pathogenicity; effector
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12030479

is the causal agent of blue mold decay on apple fruits and is also known to be the major producer of patulin, a mycotoxin that represents serious hazard to human health. Several mechanisms have been suggested to explain the pathogenesis of in host plants. Secreted effector proteins are vital for the pathogenicity of many fungal pathogens through manipulating their hosts for efficient colonization. In this study, we performed a RNA-Seq analysis followed by computational prediction of effector proteins from during infection of the host apple fruits, and a total of 212 and 268 candidate effector protein genes were identified at 6 and 9 h after inoculation (hai), respectively. One of the candidate effector protein genes was identified as a concanavalin A-like lectin/glucanase ( ), which was dramatically induced during the pathogen-host interaction. Targeted knockout of resulted in significant reduction in conidial production and germination relative to the wild type. Further studies showed that in addition to salt stress, the mutant was much more sensitive to SDS and Congo red, suggesting a defect in cell wall integrity. Pathogenicity assays revealed that the Δ mutant showed significant decrease in virulence and infectious growth on apple fruits. All these results suggest that is required for fungal growth, stress response, and the virulence of .