Gensenoside Rb1 protects rat PC12 cells from oxidative stress-induced endoplasmic reticulum stress: the involvement of thioredoxin-1

• Published in: Molecular and cellular biochemistry Vol. 410; no. 1-2; pp. 239 - 246
• Main Authors: Zeng, Xian-Si, Jia, Jin-Jing, Ma, Li-Fang
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2015; Springer; Springer Nature B.V
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis - drug effects; Biochemistry; Biomedical and Life Sciences; Cardiology; Cell Survival - drug effects; Cellular biology; Cytoprotection; Dose-Response Relationship, Drug; Endoplasmic Reticulum Stress - drug effects; Ginsenosides - pharmacology; Herbal medicine; Hydrogen peroxide; Hydrogen Peroxide - toxicity; Life Sciences; Medical Biochemistry; Neurons - drug effects; Neurons - metabolism; Neurons - pathology; Neuroprotective Agents - pharmacology; Oncology; Oxidants - toxicity; Oxidative stress; Oxidative Stress - drug effects; PC12 Cells; Phytochemicals; Protein expression; Rats; RNA Interference; Thioredoxins; Thioredoxins - genetics; Thioredoxins - metabolism; Transfection; Gensenoside Rb1; Oxidative stress; Thioredoxin-1; Endoplasmic reticulum stress
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-015-2557-1

Oxidative stress plays an important role in many diseases and hydrogen peroxide (H 2 O 2 ) plays a central role in the stress. Gensenoside Rb1 is the one of active ingredients in the traditional Chinese medicine Panax notoginseng . It has been reported that gensenoside Rb1 possesses various pharmacological activities. Here we report that gensenoside Rb1 exhibits potent protective effects against oxidative injury induced by H 2 O 2 through inhibiting endoplasmic reticulum stress in PC12 cells. Cell viability assay demonstrated that incubation with H 2 O 2 for 24 h led to a significant loss of cultured rat PC12 cells, and the cell viability was pronouncedly increased by pretreatment of gensenoside Rb1 for 24 h. H 2 O 2 -induced endoplasmic reticulum stress pathway was also suppressed after gensenoside Rb1 pretreatment, which was related with thioredoxin-1 (Trx-1) induction. Trx-1 siRNA abolished the protective effects of gensenoside Rb1. Our results of the present study demonstrate that gensenoside Rb1 shows a potent anti-oxidative effect on cultured PC12 cells by inducing Trx-1 expression.