Mapping of microsatellite loci and association of aorta atherosclerosis with LG VI markers in the rabbit

• Published in: Physiological genomics Vol. 6; no. 1; pp. 11 - 18
• Main Authors: KORSTANJE, R, GILLISSEN, G. F, KODDE, L. P, DEN BIEMAN, M, LANKHORST, A, VAN ZUTPHEN, L. F. M, VAN LITH, H. A
• Format: Journal Article
• Language: English
• Published: United States Am Physiological Soc 06.06.2001
• Subjects: Alleles; Animals; Aortic Diseases - genetics; Aortic Diseases - pathology; Arteriosclerosis - genetics; Arteriosclerosis - pathology; Chromosome Mapping; Diet, Atherogenic; DNA-Binding Proteins; Female; Genetic Linkage; Genetic Markers; Male; Microsatellite Repeats; Quantitative Trait, Heritable; Rabbits - genetics; Telomerase - genetics
• ISSN: 1094-8341; 1531-2267
• DOI: 10.1152/physiolgenomics.2001.6.1.11

Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, 3508 TD Utrecht, The Netherlands Twenty-three rabbit microsatellites were extracted from the EMBL nucleotide database. Nine of these markers, together with nine earlier published microsatellite markers, were found to be polymorphic between the AX/JU and IIIVO/JU inbred strains. By using an F 2 intercross we could integrate five markers into the rabbit linkage map. One anonymous microsatellite marker could be assigned to chromosome 1, and one microsatellite marker, located within the metallothionein-1 gene, could be added to linkage group VI (LG VI). Three microsatellite markers (one anonymous, one located within the PMP2 gene, and one located within the FABP6 gene) constitute a new linkage group (LG XI). We also measured the degree of dietary cholesterol-induced aorta atherosclerosis in the F 2 animals. A significant cosegregation was found between the degree of aorta atherosclerosis and the allelic variation of the biochemical marker Est-2 on LG VI in male rabbits. This association was not found in female rabbits. atherosclerosis; genetic map; quantitative trait locus; microsatellite