Genome-Wide Analysis of the Soybean Calmodulin-Binding Protein 60 Family and Identification of GmCBP60A-1 Responses to Drought and Salt Stresses

• Published in: International journal of molecular sciences Vol. 22; no. 24; p. 13501
• Main Authors: Yu, Qian, Liu, Ya-Li, Sun, Guo-Zhong, Liu, Yuan-Xia, Chen, Jun, Zhou, Yong-Bin, Chen, Ming, Ma, You-Zhi, Xu, Zhao-Shi, Lan, Jin-Hao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.12.2021; MDPI
• Subjects: Abiotic stress; Arabidopsis; Arabidopsis - genetics; Calcium-binding protein; Calmodulin; Calmodulin-Binding Proteins - genetics; CBP60 proteins; Chromosomes; DNA methylation; Drought; drought tolerance; Droughts; Functional analysis; Gene expression; Gene Expression Regulation, Plant - genetics; Genome-Wide Association Study - methods; Genomes; Germination; Glycine max - genetics; Hairy root; hairy root assay; Kinases; Localization; Malondialdehyde; Maximum likelihood method; Phylogenetics; Physiology; Plant growth; Plant Proteins - genetics; Plants, Genetically Modified - genetics; Proline; Proteins; RNA-mediated interference; Salinity tolerance; Salt Stress - genetics; salt tolerance; Salt Tolerance - genetics; Seedlings; Seedlings - genetics; Seeds; Seeds - genetics; soybean; Soybean Proteins - genetics; Soybeans; Stress, Physiological - genetics; Water loss; soybean; salt tolerance; drought tolerance; CBP60 proteins; Arabidopsis; hairy root assay
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222413501

Calmodulin-binding protein 60 (CBP60) members constitute a plant-specific protein family that plays an important role in plant growth and development. In the soybean genome, nineteen CBP60 members were identified and analyzed for their corresponding sequences and structures to explore their functions. Among GmCBP60A-1, which primarily locates in the cytomembrane, was significantly induced by drought and salt stresses. The overexpression of enhanced drought and salt tolerance in , which showed better state in the germination of seeds and the root growth of seedlings. In the soybean hairy roots experiment, the overexpression of increased proline content, lowered water loss rate and malondialdehyde (MDA) content, all of which likely enhanced the drought and salt tolerance of soybean seedlings. Under stress conditions, drought and salt response-related genes showed significant differences in expression in hairy root soybean plants of -overexpressing and hairy root soybean plants of RNAi. The present study identified as an important gene in response to salt and drought stresses based on the functional analysis of this gene and its potential underlying mechanisms in soybean stress-tolerance.