Elicitation of Suspension-Cultured Tomato Cells Triggers the Formation of Phosphatidic Acid and Diacylglycerol Pyrophosphate

• Published in: Plant physiology (Bethesda) Vol. 123; no. 4; pp. 1507 - 1515
• Main Authors: Arnold H. van der Luit, Titus Piatti, Aveline van Doorn, Musgrave, Alan, Felix, Georg, Boller, Thomas, Munnik, Teun
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.08.2000
• Subjects: Animal cells; Biological and medical sciences; Cell Biology and Signal Transduction; Cell physiology; Cells, Cultured; Chromatography, Thin Layer; Diglycerides; Diphosphates - metabolism; Flagellin - pharmacology; Fundamental and applied biological sciences. Psychology; Generalities. Disease free stocks; Glycerol - analogs & derivatives; Glycerol - metabolism; Hydrolysis; Kinetics; Lipids; Lycopersicon esculentum - cytology; Lycopersicon esculentum - metabolism; Lycopersicon esculentum - physiology; Molecular and cellular biology; Oligosaccharides - pharmacology; Peptide Fragments - pharmacology; Phosphatidic acids; Phosphatidic Acids - biosynthesis; Phosphatidic Acids - metabolism; Phosphatidylinositol phosphates; Phospholipids; Phospholipids - metabolism; Phytopathology. Animal pests. Plant and forest protection; Plant cells; Plants; Signal transduction; Signal Transduction - physiology; Space life sciences; Xylan Endo-1,3-beta-Xylosidase; Xylosidases - pharmacology; Cell culture; Enzyme; Transferases; Diacylglycerol; Host agent relation; Esterases; Biosynthesis; Phosphoric diester hydrolases; Diacylglycerol kinase; Phospholipase C; Signal transduction; Enzymatic activity; Dicotyledones; Angiospermae; Lycopersicon esculentum; Hydrolases; Defense mechanism; Spermatophyta; Elicitation; Solanaceae; Diphosphates; Experimental plant; Biological accumulation; Phosphatidic acid
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.123.4.1507

Phosphatidic acid (PA) and its phosphorylated derivative diacylglycerol pyrophosphate (DGPP) are lipid molecules that have been implicated in plant cell signaling. In this study we report the rapid but transient accumulation of PA and DGPP in suspension-cultured tomato (Lycopersicon esculentum) cells treated with the general elicitors, N,N′,N″,N″′-tetraacetyl-chitotetraose, xylanase, and the flagellin-derived peptide flg22. To determine whether PA originated from the activation of phospholipase D or from the phosphorylation of diacylglycerol (DAG) by DAG kinase, a strategy involving differential radiolabeling with [32P]orthophosphate was used. DAG kinase was found to be the dominant producer of PA that was subsequently metabolized to DGPP. A minor but significant role for phospholipase D could only be detected when xylanase was used as elicitor. Since PA formation was correlated with the high turnover of polyphosphoinositides, we hypothesize that elicitor treatment activates phospholipase C to produce DAG, which in turn acts as substrate for DAG kinase. The potential roles of PA and DGPP in plant defense signaling are discussed.