In vivo screen of genetically conserved Streptococcus pneumoniae proteins for protective immunogenicity

• Published in: Vaccine Vol. 34; no. 50; pp. 6292 - 6300
• Main Authors: Anderson, Richard J, Guru, Siradanahalli, Weeratna, Risini, Makinen, Shawn, Falconer, Derek J, Sheppard, Neil C, Lang, Susanne, Chang, Bingsheng, Goenaga, Anne-Laure, Green, Bruce A, Merson, James R, Gracheck, Stephen J, Eyles, Jim E
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 07.12.2016; Elsevier Limited
• Subjects: Allergy and Immunology; Animals; Antibody; Antigen; Antigens; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacteria; Bacterial Load; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Cloning; Cloning, Molecular; Computational Biology; Conserved Sequence; Disease Models, Animal; Escherichia coli; Escherichia coli - genetics; Experiments; Female; Gene Expression; Genetic Testing; Immunity; Immunization; Immunogenicity; Laboratory animals; Lipoproteins; Lung - microbiology; Mice, Inbred BALB C; Mice, Inbred CBA; Pneumococcal; Pneumococcus; Pneumonia; Pneumonia, Pneumococcal - microbiology; Pneumonia, Pneumococcal - prevention & control; Proteins; Streptococcus infections; Streptococcus pneumoniae; Streptococcus pneumoniae - genetics; Streptococcus pneumoniae - immunology; Survival Analysis; Vaccine; Vaccines; United States > US; Pneumococcal; Antigen; Vaccine; Antibody; Pneumococcus; Immunity
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2016.10.061

Highlights • Immunizing efficacy of 52 pneumococcal proteins evaluated in a mouse challenge model. • Proteins were selected based on genetic conservation and surface accessibility to antibody. • Anti-sera used to investigate antigen expression across multiple serotypes. • Seven protective antigens were evaluated in additional animal models. • Protective antigens were Histidine triad’s or proteins bearing LPXTG linkage domains.