Genome-Wide Identification and Expression Analysis of BraGLRs Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 23; p. 3729
• Main Authors: Yang, Liu, Zhao, Yumei, Wu, Xiaoyu, Zhang, Yang, Fu, Yehan, Duan, Qiaohong, Ma, Wei, Huang, Jiabao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.11.2022; MDPI
• Subjects: Abiotic stress; Bioinformatics; Biosynthesis; Brassica rapa; Brassica rapa - metabolism; Cell division; Drought; expression profile; Flowers; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genomes; GLR gene family; Glutamic acid receptors; Homeostasis; Life cycles; Ligands; Localization; Low temperature; Methyl jasmonate; Mutants; Phylogenetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plant reproduction; Pollination; Proteins; Receptor channels; Salicylic acid; Sexual reproduction; Signal transduction; Stress, Physiological - genetics; Trees; China; sexual reproduction; Brassica rapa; GLR gene family; expression profile; abiotic stress
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11233729

Glutamate receptors (GLRs) are involved in multiple functions during the plant life cycle through affecting the Ca concentration. However, GLRs in Brassica species have not yet been reported. In this study, 16 glutamate receptor-like channels (GLR) belonged to two groups were identified in the ( ) genome by bioinformatic analysis. Most members contain domains of ANF_receptor, Peripla_BP_6, Lig_chan, SBP_bac_3, and Lig_chan_Glu_bd that are closely related to glutamate receptor channels. This gene family contains many elements associated with drought stress, low temperature stress, methyl jasmonate (MeJA), salicylic acid (SA), and other stress resistance. Gene expression profiles showed that genes were expressed in roots, stems, leaves, flowers, and siliques. expression was elevated after drought stress in drought-sensitive plants. , , and expression were significantly upregulated after salt stress. expression is higher in the female sterile-line mutants than in the wild type. The expression levels of , , , and were significantly higher in the male sterile-line mutants than in the wild type. The expression of most increased after self-pollination, with exhibiting the greatest increase. These results suggest that play an important role in abiotic stress tolerance and sexual reproduction.