Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression

• Published in: International journal of molecular sciences Vol. 22; no. 22; p. 12135
• Main Authors: Kurianowicz, Katarzyna, Klatka, Maria, Polak, Agnieszka, Hymos, Anna, Bębnowska, Dominika, Podgajna, Martyna, Hrynkiewicz, Rafał, Sierawska, Olga, Niedźwiedzka-Rystwej, Paulina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.11.2021; MDPI
• Subjects: Adolescent; Antigens, CD19 - genetics; Antigens, CD19 - immunology; Autoimmune diseases; B-Lymphocytes - immunology; Beta cells; CD19 antigen; CD3 antigen; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - pathology; Child; Child, Preschool; children; Diabetes; Diabetes mellitus (insulin dependent); Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - immunology; Diabetes Mellitus, Type 1 - pathology; Disease; Female; Gene Expression Regulation - genetics; Humans; Immune system; Immunity, Innate - genetics; Immunity, Innate - immunology; Immunological tolerance; Innate immunity; Insulin; Interleukin 10; Interleukin-10 - genetics; Interleukin-10 - immunology; Killer Cells, Natural - immunology; Killer Cells, Natural - metabolism; Lymphocytes; Lymphocytes T; Male; Metabolic disorders; Pathogenesis; Pathogens; Patients; Pediatrics; Proteins; TLR2 protein; TLR4 protein; TLR9 protein; Toll-Like Receptor 2 - genetics; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 9 - genetics; Toll-like receptors; Toll-Like Receptors - genetics; Toll-Like Receptors - immunology; type 1 diabetes mellitus; Toll-like receptors; type 1 diabetes mellitus; children
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222212135

Type 1 diabetes (DM1) is classified as an autoimmune disease. An uncontrolled response of B and T lymphocytes to the body's own tissues develops in the absence of immune tolerance. The main aim of the study was to evaluate the effect of the duration of type 1 diabetes in children on the expression of TLR receptors and the relationship with the parameters of glycemic control in patients. As a result, we showed significant differences in the level of TLR2, TLR4 and TLR9 expression in patients with DM1 in the early stage of the disease and treated chronically compared to the healthy group. Additionally, in this study, we found that the numbers of CD19+ B cells, CD3+ CD4+, CD3+ CD8+ T cells and NK cells are different for newly diagnosed DM1 individuals, patients receiving chronic treatment and for healthy controls, indicating an important role of these cells in killing pancreatic beta cells. Moreover, higher levels of IL-10 in patients with newly diagnosed DM1 have also been found, confirming the reports found in the literature.