Suppression of dust mite extract and 2,4-dinitrochlorobenzene-induced atopic dermatitis by the water extract of Lindera obtusiloba

• Published in: Journal of ethnopharmacology Vol. 137; no. 1; pp. 802 - 807
• Main Authors: Choi, Eun-Ju, Lee, Soyoung, Kim, Hui-Hun, Singh, Thoudam S.K., Choi, Jin Kyeong, Choi, Hyun Gyu, Suh, Won Mo, Lee, Seung-Ho, Kim, Sang-Hyun
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.09.2011; Elsevier
• Subjects: Administration, Cutaneous; Animal models; Animals; Anti-Allergic Agents - administration & dosage; Anti-Allergic Agents - chemistry; Anti-Allergic Agents - isolation & purification; Anti-Allergic Agents - pharmacology; Antigens, Dermatophagoides; Atopic dermatitis; Biological and medical sciences; Dermatitis, Atopic - chemically induced; Dermatitis, Atopic - genetics; Dermatitis, Atopic - immunology; Dermatitis, Atopic - pathology; Dermatitis, Atopic - prevention & control; Dermatophagoides; Dermatophagoides farinae; Dermatophagoides pteronyssinus; Dinitrochlorobenzene; Disease Models, Animal; Dose-Response Relationship, Drug; Dust; Ear; Female; Gene expression; Gene Expression Regulation - drug effects; General pharmacology; Histamine; Histamine - blood; IgE; Immunoglobulin E; Immunoglobulin E - blood; Inflammation; Interleukin 13; Interleukin 4; Interleukin-13 - genetics; Interleukin-4 - genetics; Interleukins - genetics; Lindera; Lindera - chemistry; Lindera obtusiloba; Mast cell; Mast cells; Mast Cells - drug effects; Mast Cells - immunology; Medical sciences; Metastases; Mice; Mice, Inbred BALB C; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plants, Medicinal; Skin - drug effects; Skin - immunology; Skin - pathology; Skin diseases; Skin inflammation; Solvents - chemistry; Time Factors; Topical application; Tumor necrosis factor; Tumor Necrosis Factor-alpha - genetics; Water - chemistry; LOWE; DFE; Skin inflammation; AD; IL; Atopic dermatitis; IgE; DNCB; Lindera obtusiloba; Mast cell; Allergy; Immunopathology; Skin disease; Pharmacognosy; Extract; Inflammation; Atopy; Medicinal plant; Dust; Dicotyledones; Angiospermae; Plant origin; Spermatophyta; Skin; Lauraceae
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2011.06.043

The Lindera obtusiloba has been used in traditional medicine for the treatment of inflammation and dermatitis. In this study, we investigated the effect of topical application of Lindera obtusiloba water extract (LOWE) on the house dust mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD). We established AD model in BALB/c mice by repeated local exposure of DFE/DNCB to the ears. After a topical application of LOWE on the skin lesions, the epidermal thickness, mast cell infiltration, and serum immunoglobulin E (IgE) and histamine were measured. In addition, the gene expression of interleukin (IL)-4, IL-13, IL-31, and tumor necrosis factor (TNF)-α in the ears was assayed. LOWE reduced AD symptoms based on ear thickness, histopathological analysis, and serum IgE levels. LOWE inhibited mast cell infiltration into the ear and elevation of serum histamine in AD model. Moreover, LOWE suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-31, and TNF-α in the ears. Our results showed that topical application of LOWE exerts beneficial effects in AD symptoms, suggesting that LOWE might be a candidate for the treatment of AD.