An electrochemical investigation of glucose oxidase at a CdS nanoparticles modified electrode

• Published in: Biosensors & bioelectronics Vol. 21; no. 5; pp. 817 - 821
• Main Authors: Huang, Yinxi, Zhang, Wenjun, Xiao, Han, Li, Genxi
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.11.2005; Elsevier Science
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Cadmium Compounds - chemistry; CdS nanoparticles; Coated Materials, Biocompatible - analysis; Coated Materials, Biocompatible - chemistry; Electrochemistry - instrumentation; Electrochemistry - methods; Electron-transfer; Enzymatic activity; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Glucose - analysis; Glucose - chemistry; Glucose oxidase; Glucose Oxidase - analysis; Glucose Oxidase - chemistry; Materials Testing; Methods. Procedures. Technologies; Microelectrodes; Nanostructures - chemistry; Nanostructures - ultrastructure; Particle Size; Sulfides - chemistry; Various methods and equipments; Glucose oxidase; Enzymatic activity; CdS nanoparticles; Electron-transfer; Biosensor; Enzyme; Nanoparticle; Electrochemistry; Electron transfer; Oxidoreductases; Enzyme electrode
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2005.01.012

The direct electrochemistry of glucose oxidase (GOD) adsorbed on a CdS nanoparticles modified pyrolytic graphite electrode was investigated, where the enzyme demonstrated significantly enhanced electron-transfer reactivity. GOD adsorbed on CdS nanoparticles maintained its bioactivity and structure, and could electrocatalyze the reduction of dissolved oxygen, which resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection. Performance and characteristics of the fabricated glucose biosensor were assessed with respect to detection limit, sensitivity, storage stability and interference exclusion. The results showed that the fabricated biosensor was sensitive and stable in detecting glucose, indicating that CdS nanoparticle was a good candidate material for the immobilization of enzyme in glucose biosensor construction.