hnRNPA2B1-Mediated Extracellular Vesicles Sorting of miR-122-5p Potentially Promotes Lung Cancer Progression

• Published in: International journal of molecular sciences Vol. 22; no. 23; p. 12866
• Main Authors: Li, Chuang, Qin, Fang, Wang, Wei, Ni, Yifan, Gao, Mingyu, Guo, Mingxiong, Sun, Guihong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.11.2021; MDPI
• Subjects: A549 Cells; Adenocarcinoma - diagnosis; Adenocarcinoma - metabolism; Adenocarcinoma - mortality; Binding sites; Disease Progression; Extracellular vesicles; Extracellular Vesicles - metabolism; Gene expression; Hepatocytes; Heterogeneous-Nuclear Ribonucleoprotein Group A-B - metabolism; hnRNPA2B1; Humans; Liver Neoplasms - metabolism; Liver Neoplasms - secondary; Lung cancer; Lung Neoplasms - diagnosis; Lung Neoplasms - metabolism; Lung Neoplasms - mortality; Metastases; Metastasis; MicroRNAs; MicroRNAs - metabolism; miR-122-5p; non-small cell lung cancer; Non-small cell lung carcinoma; Prognosis; Protein transport; Proteins; RNA-binding protein; Roles; Tumor cells; Tumors; Vesicles; China; non-small cell lung cancer; RNA-binding protein; miR-122-5p; extracellular vesicles; hnRNPA2B1
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222312866

Extracellular vesicles (EVs) released by tumor cells play important roles on the remodeling of the tumor-stromal environment and on promoting tumor metastasis. Our earlier studies revealed that miR-122-5p, a type of small non-coding RNA, was dysregulated in non-small cell lung cancer (NSCLC) cell-derived EVs. In this study, we found that miR-122-5p was selectively sorted and secreted into lung cancer EVs through binding to RNA-binding protein hnRNPA2B1. In addition, we found that hnRNPA2B1 interacted with miR-122-5p through the EXO-motif. The delivering of lung cancer EVs-miR-122-5p promoted the migration of liver cells, which may play roles in establishing a pre-metastatic micro-environment and hepatic metastasis of lung cancer. Importantly, our findings revealed the molecular mechanism that RNA-binding protein controls the selective sorting of tumor-derived EV miR-122-5p, which potentially promotes lung cancer progression.