Integrated Transcriptome and Metabolome Analysis to Identify Sugarcane Gene Defense against Fall Armyworm ( Spodoptera frugiperda) Herbivory

• Published in: International journal of molecular sciences Vol. 23; no. 22; p. 13712
• Main Authors: Li, Ao-Mei, Wang, Miao, Chen, Zhong-Liang, Qin, Cui-Xian, Liao, Fen, Wu, Zhen, He, Wei-Zhong, Lakshmanan, Prakash, Pan, You-Qiang, Huang, Dong-Liang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.11.2022; MDPI
• Subjects: Amidase; Amino acids; Amino Acids - genetics; Animals; Annotations; Association analysis; Biosynthesis; Breeding; Carbohydrate metabolism; Carbohydrates; Correlation coefficients; Cytochrome; Edible Grain - genetics; Fatty acids; Flavonoids; Gene expression; Genes; Heat shock proteins; Herbivory; Insecta - physiology; Insects; Kinases; Larvae; Lipoxygenase; Metabolism; Metabolites; Metabolome; Metabolomics; Oxidative stress; Pathogens; Peroxidase; Pest resistance; Pests; plant defense; Plant Growth Regulators; Protein biosynthesis; RNA sequencing; Saccharum - genetics; Secondary metabolites; Signal transduction; Spodoptera - genetics; Spodoptera frugiperda; Sugarcane; Sugars; Transcription factors; Transcriptome; Transcriptomes; VOCs; Volatile organic compounds; RNA sequencing; Spodoptera frugiperda; metabolism; plant defense; sugarcane
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232213712

Sugarcane is the most important sugar crop, contributing ≥80% to total sugar production around the world. is one of the main pests of sugarcane, potentially causing severe yield and sugar loss. The identification of key defense factors against herbivory can provide targets for improving sugarcane resistance to insect pests by molecular breeding. In this work, we used one of the main sugarcane pests, , as the tested insect to attack sugarcane. Integrated transcriptome and metabolomic analyses were performed to explore the changes in gene expression and metabolic processes that occurred in sugarcane leaf after continuous herbivory by larvae for 72 h. The transcriptome analysis demonstrated that sugarcane pest herbivory enhanced several herbivory-induced responses, including carbohydrate metabolism, secondary metabolites and amino acid metabolism, plant hormone signaling transduction, pathogen responses, and transcription factors. Further metabolome analysis verified the inducement of specific metabolites of amino acids and secondary metabolites by insect herbivory. Finally, association analysis of the transcriptome and metabolome by the Pearson correlation coefficient method brought into focus the target defense genes against insect herbivory in sugarcane. These genes include and in amino acid metabolism, in phenylpropanoid biosynthesis, and in plant hormone signal transduction. A putative regulatory model was proposed to illustrate the sugarcane defense mechanism against insect attack. This work will accelerate the dissection of the mechanism underlying insect herbivory in sugarcane and provide targets for improving sugarcane variety resistance to insect herbivory by molecular breeding.