Interaction of human plasma fibronectin with viral proteins of human immunodeficiency virus

• Published in: FEMS immunology and medical microbiology Vol. 8; no. 2; pp. 127 - 131
• Main Authors: Torre, Donato, Pugliese, Agostino, Ferrario, Giulio, Marietti, Giorgio, Forno, Brunella, Zeroli, Claudia
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.1994; Blackwell
• Subjects: AIDS/HIV; Biological and medical sciences; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Fibronectin; Fibronectin binding to HIV‐1; Fibronectins - blood; Fibronectins - immunology; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; HIV Envelope Protein gp120 - metabolism; HIV Envelope Protein gp41 - metabolism; HIV-1 - metabolism; HIV‐1; human immunodeficiency virus; Humans; Microbiology; Oligopeptides - metabolism; Peptide Fragments - metabolism; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Virology; Virus; Human; Proteins; Plasma; HIV-1 virus; Retroviridae; Lentivirinae; Molecular interaction; Glycoproteins; Human immunodeficiency virus; Infected cell; Fibronectin
• ISSN: 0928-8244; 1574-695X
• DOI: 10.1111/j.1574-695X.1994.tb00434.x

Fibronectin (FN) is present in soluble and matrix forms in various body fluids and tissues, and has been shown to bind to several pathogens, including viruses. The interaction of FN with viral proteins of human immunodeficiency virus (HIV‐1) was investigated by immunofluorescence technique using a cell line chronically infected with HIV‐1 (H9‐V). The results of this study showed that FN binds to HIV‐1 infected cells. especially at FN concentration of 5 μg/ml. In addition, FN‐pentapeptide has shown the ability to bind to HIV‐1 infected cells. On the other hand, preincubation with antibodies against FN abolished the binding of FN to HIV‐1 infected cells. Finally, FN has shown to bind to HIV‐1 glycoproteins, including gp41 and pg120. In contrast, no binding to HIV‐1 core proteins, including p15 and p24, was noted. We suggest that FN, in binding HIV‐1 particles, may reduce viremia and thus may be involved in the clearance of viral proteins from the cells.