The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis

• Published in: Electrophoresis Vol. 16; no. 6; p. 1002
• Main Authors: Ueda, T, Maekawa, T, Sadamitsu, D, Oshita, S, Ogino, K, Nakamura, K
• Format: Journal Article
• Language: English
• Published: Germany 1995
• Subjects: Electrophoresis, Capillary - methods; Humans; Nitrates - blood; Nitrites - blood; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 0173-0835
• DOI: 10.1002/elps.11501601167

The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis (CZE) is described. Electrophoresis was carried out in a 65 cm long, 75 microns wide silica capillary, using 750 mM sodium chloride containing 5% NICE-Pak OFM Anion-BT (an additive reversing the electroosmotic flow) as running buffer, at a potential of 20 kV, with on-column UV detection at 214 nm. Under these conditions, the two anions migrated in the order of nitrite and nitrate, completely resolved from each other. Forty-one samples of ultrafiltered blood plasma of healthy volunteers were analyzed. The concentrations of nitrite and nitrate were 0.15 +/- 0.07 mg/L and 3.2 +/- 1.6 mg/L (mean +/- SD), respectively, with a linear correlation in the range of 0.1-50 mg/L. The within-day and between-day coefficients of variation (CVs) for nitrite and nitrate were lower than 5% and 8%, respectively. The recovery was 97-114%. The estimated plasma volume needed for each determination was 108 nL.