Genotypic diversity and migration of clonal lineages of Botrytis cinerea from chickpea fields of Bangladesh inferred by microsatellite markers

• Published in: Plant pathology Vol. 57; no. 5; pp. 967 - 973
• Main Authors: Isenegger, D.A, Macleod, W.J, Ford, R, Taylor, P.W.J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Biological and medical sciences; Botryotinia fuckeliana; Botrytis cinerea; botrytis grey mould; Cicer arietinum; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; genetic diversity; genetic variation; molecular markers; Phytopathology. Animal pests. Plant and forest protection; population structure; Sclerotinia fuckeliana; Bangladesh; Asia; Indian Peninsula; South Asia; Botryotinia fuckeliana; Plant pathology; Plant pathogen; Botrytis cinerea; Tropical zone; Migration; Molecular marker; Biodiversity; Fungi; Field; Cicer arietinum; Dicotyledones; Microsatellite DNA; Angiospermae; Mould; Fungi Imperfecti; Ascomycota; botrytis grey mould; Population structure; Genetic diversity; Chick pea; Grain legume; Leguminosae; Spermatophyta; molecular markers
• ISSN: 0032-0862; 1365-3059
• DOI: 10.1111/j.1365-3059.2008.01885.x

An analysis of allelic diversity at nine microsatellite loci provided an insight into the population structure of Botrytis cinerea from four fields (sampled in 2003 and 2004) that represented important regional locations for chickpea production in Bangladesh. Although three populations were limited by sample size after clone-correction, a total of 51 alleles were amplified among 146 B. cinerea isolates from Bangladesh, which revealed a high amount of within-population and overall genetic diversity (HS = 0·48 and HT = 0·54, respectively). The percentage of maximal genotypic diversity (G) ranged between populations (G = 23-40), with a total of 69 haplotypes detected (G = 25). Bayesian cluster analysis depicted two major clusters distributed among the four Bangladesh populations, indicating population admixture from two origins that have spread throughout these regions. Genotype flow between regions was detected and indicated the spread of clonal lineages, consistent with relatively low differentiation among the four populations (mean GST = 0·1, P < 0·05). These results highlighted the potential threat of host resistance breakdown as a result of considerable genetic diversity, genotype flow and the evolutionary potential of B. cinerea.