The extracellular matrix protein mindin regulates trafficking of murine eosinophils into the airspace

• Published in: Journal of leukocyte biology Vol. 85; no. 1; pp. 124 - 131
• Main Authors: Li, Zhuowei, Garantziotis, Stavros, Jia, Wei, Potts, Erin N., Lalani, Sikander, Liu, Zhi, He, You‐Wen, Foster, W. Michael, Hollingsworth, John W.
• Format: Journal Article
• Language: English
• Published: England Society for Leukocyte Biology 01.01.2009; The Society for Leukocyte Biology
• Subjects: Animals; asthma; Asthma - immunology; Asthma - pathology; Cell Adhesion - physiology; Cell Movement; environmental airways disease; Eosinophils - physiology; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - physiology; Extracellular Mediators and Effector Molecules; Hypersensitivity - immunology; Hypersensitivity - pathology; IL‐13; Immunoglobulin E - metabolism; In Vitro Techniques; integrin; Integrin alpha4beta1 - metabolism; Interleukin-13 - metabolism; Interleukin-4 - metabolism; Lung - drug effects; Lung - immunology; Lung - pathology; Macrophage-1 Antigen - metabolism; Methacholine Chloride - pharmacology; Mice; Mice, Inbred C57BL; Mice, Transgenic; Ovalbumin - immunology; spondin2
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1189/jlb.0208135

Asthma remains a major cause of morbidity and hospitalizations in developed nations. Despite the widespread prevalence of this disease, the genetic and environmental factors that mediate development and progression of allergic airways disease remain poorly understood. Pulmonary recruitment of eosinophils is believed to contribute to many cardinal features of allergic airways disease. Therefore, it is paramount to understand host factors that contribute to pulmonary eosinophil recruitment into the lungs. Mindin is a component of pulmonary extracellular matrix, which can regulate inflammatory cell recruitment. We characterized the role of mindin in the severity of allergic airways disease using established murine models. There were no baseline differences in wild‐type and mindin‐deficient animals in cell counts or airway physiology. Using the OVA murine model of allergic airways disease, we observed that mindin‐deficient animals have less‐severe allergic airways disease with fewer airspace eosinophils and lower lung‐lavage levels of inflammatory Th2 cytokines such as IL‐13 and IL‐4. Furthermore, mindin‐deficient animals have reduced airway hyper‐responsiveness after methacholine challenge. To determine the role of mindin in eosinophil trafficking, independent of antigen immunization or T lymphocyte activation, we instilled IL‐13 directly into the lungs of mice. In this model, mindin regulates eosinophil recruitment into the airspace. In vitro experiments demonstrate that mindin can enhance eotaxin‐mediated eosinophil adhesion and migration, which are dependent on the expression of integrins αMβ2 and α4β1. In conclusion, these data suggest that mindin participates in integrin‐dependent trafficking of eosinophils and can contribute to the severity of allergic airways disease.