Nonivamide Enhances miRNA let-7d Expression and Decreases Adipogenesis PPARγ Expression in 3T3-L1 Cells

• Published in: Journal of cellular biochemistry Vol. 116; no. 6; pp. 1153 - 1163
• Main Authors: Rohm, Barbara, Holik, Ann-Katrin, Kretschy, Nicole, Somoza, Mark M., Ley, Jakob P., Widder, Sabine, Krammer, Gerhard E., Marko, Doris, Somoza, Veronika
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.06.2015; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Subjects: 3T3-L1 ADIPOGENESIS; 3T3-L1 Cells; Adipogenesis; Adipogenesis - drug effects; Animals; Capsaicin; Capsaicin - analogs & derivatives; Capsaicin - pharmacology; Capsaicin receptors; Cell activation; CELL DIFFERENTIATION; Diet; Dietary intake; Energy intake; Energy metabolism; Food intake; Ion channels; LIPID ACCUMULATION; Lipids; Mice; microRNA; MicroRNAs - metabolism; miRNA; PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR (PPAR); Peroxisome proliferator-activated receptors; PPAR gamma - metabolism; Receptor mechanisms; Receptors; trans-tert-BUTYLCYCLOHEXANOL; Transient receptor potential proteins; Triglycerides; TRPV Cation Channels - metabolism; TRPV1; 3T3-L1 ADIPOGENESIS; trans-tert-BUTYLCYCLOHEXANOL; LIPID ACCUMULATION; CELL DIFFERENTIATION; microRNA; PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR (PPAR); TRPV1
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.25052

ABSTRACT Red pepper and its major pungent principle, capsaicin (CAP), have been shown to be effective anti‐obesity agents by reducing energy intake, enhancing energy metabolism, decreasing serum triacylglycerol content, and inhibiting adipogenesis via activation of the transient receptor potential cation channel subfamily V member 1 (TRPV1). However, the binding of CAP to the TRPV1 receptor is also responsible for its pungent sensation, strongly limiting its dietary intake. Here, the effects of a less pungent structural CAP‐analog, nonivamide, on adipogenesis and underlying mechanisms in 3T3‐L1 cells were studied. Nonivamide was found to reduce mean lipid accumulation, a marker of adipogenesis, to a similar extent as CAP, up to 10.4% (P < 0.001). Blockage of the TRPV1 receptor with the specific inhibitor trans‐tert‐butylcyclohexanol revealed that the anti‐adipogenic activity of nonivamide depends, as with CAP, on TRPV1 receptor activation. In addition, in cells treated with nonivamide during adipogenesis, protein levels of the pro‐adipogenic transcription factor peroxisome‐proliferator activated receptor γ (PPARγ) decreased. Results from miRNA microarrays and digital droplet PCR analysis demonstrated an increase in the expression of the miRNA mmu‐let‐7d‐5p, which has been associated with decreased PPARγ levels. J. Cell. Biochem. 116: 1153–1163, 2015. © 2015 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc.