Effects of the Flavanone combination Hesperetin‐Naringenin, and Orange and Grapefruit Juices, on Airway inflammation and Remodeling in a murine asthma model

• Published in: Phytotherapy research Vol. 29; no. 4; pp. 591 - 598
• Main Authors: Seyedrezazadeh, Ensiyeh, Kolahian, Saeed, Shahbazfar, Amir‐Ali, Ansarin, Khalil, Pour Moghaddam, Masoud, Sakhinia, Masoud, Sakhinia, Ebrahim, Vafa, Mohammadreza
• Format: Journal Article
• Language: English
• Published: England Heyden & Son 01.04.2015; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: Airway Remodeling - drug effects; animal disease models; Animals; asthma; Asthma - drug therapy; Asthma - pathology; Beverages; Bronchoalveolar Lavage Fluid - cytology; Citrus paradisi - chemistry; Citrus sinensis - chemistry; Disease Models, Animal; dust; dust mites; Eosinophils - cytology; fibrosis; Flavanones - pharmacology; Fruit - chemistry; goblet cells; grapefruit juice; hesperetin; Hesperidin - pharmacology; histopathology; hypertrophy; inflammation; Inflammation - drug therapy; Lung - pathology; macrophages; Macrophages - cytology; Male; metaplasia; Mice; Mice, Inbred BALB C; naringenin; orange juice; remodeling; smooth muscle; naringenin; grapefruit juice; orange juice; remodeling; asthma; hesperetin
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.5292

We investigated whether flavanones, hesperetin–naringenin, orange, and grapefruit juices reduce airway inflammation and remodeling in murine chronic asthma model. To establish chronic asthma, mice received house dust mite (HDM) for 3 days in 2 weeks, followed by twice per week for 4 weeks. Concurrently, during the last 4 weeks, mice received hesperetin plus naringenin (HN), orange plus grapefruit juice (OGJ), orange juice (OJ), or grapefruit juice (GJ); whereas the asthmatic control (AC) group and non‐asthmatic control (NC) group consumed water ad libitum. In histopathological examination, no goblet cells metaplasia was observed in the HN, OJ, and GJ groups; also, intra‐alveolar macrophages decreased compared with those of the AC group. Hesperetin plus naringenin significantly decreased subepithelial fibrosis, smooth muscle hypertrophy in airways, and lung atelectasis compared with the AC group. Also, there was a reduction of subepithelial fibrosis in airways in OJ and GJ groups compared with AC group, but it was not noticed in OGJ group. In bronchoalveolar lavage fluid, macrophages numbers decreased in OJ and OGJ groups, whereas eosinophil numbers were increased in OJ group compared with NC group. Our finding revealed that hesperetin plus naringenin ameliorate airway structural remodeling more than orange juice and grapefruit juice in murine model of HDM‐induced asthma. Copyright © 2015 John Wiley & Sons, Ltd.