Effect of phenolic monomers on biomass and hydrolytic enzyme activities of an anaerobic fungus isolated from wild nil gai (Baselophus tragocamelus)

• Published in: Letters in applied microbiology Vol. 36; no. 6; pp. 377 - 381
• Main Authors: Paul, S.S., Kamra, D.N., Sastry, V.R.B., Sahu, N.P., Kumar, A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.2003; Blackwell Science
• Subjects: Animals; Animals, Wild - microbiology; Antelopes - microbiology; Baselophus tragocamelus; Biological and medical sciences; Biomass; Catechols - pharmacology; Coumaric Acids - pharmacology; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Hydrolases - metabolism; hydrolytic enzymes; Male; Microbiology; nil gai; phenolic monomers; Phenols - chemistry; Phenols - pharmacology; Piromyces - drug effects; Piromyces - enzymology; Piromyces - growth & development; Piromyces - isolation & purification; Piromyces sp; Rumen - microbiology; Vanillic Acid - pharmacology; Fungi; Enzymatic activity; Anaerobe; Applied microbiology; Phenols; Biomass; Thallophyta
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1046/j.1472-765X.2003.01331.x

Aims: To test the anaerobic fungus, Piromyces sp. FNG5, for its tolerance to phenolic monomers released in the rumen by degradation of lignocellulosic poor‐quality feeds. Methods and Results: Effects of phenolic monomers on biomass and fibrolytic enzyme activities of a pure culture of lignocellulolytic anaerobic fungus (Piromyces sp. FNG5) isolated from faeces of wild nil gai (blue bull, Baselophus tragocamelus) were evaluated. There was a reduction in fungal biomass at 1 mm concentration of catechol with complete inhibition at 10 mm. p‐Coumaric acid caused a reduction in biomass at 10 mm and no growth was observed above 20 mm concentration. The fungal isolate could tolerate up to 5 mm of ferulic acid without any reduction in biomass level, and was able to grow to some extent up to the highest level of ferulic acid tested (20 mm). Vanillic acid had no effect on biomass of the fungus even up to 50 mm level. The phenolic monomers varied in their potential to inhibit the secretion of carboxymethyl cellulase, xylanase, β‐glucosidase and acetyl esterase activities with catechol being the most inhibitory and vanillic acid being the least inhibitory. After 14 days of incubation, 38·49–65·14%p‐Coumaric acid, 65·22–74·10% ferulic acid and 34·13–66·78% vanillic acid disappeared from the medium under anaerobic conditions. Conclusions, Significance and Impact of the Study: It is concluded that the anaerobic fungus Piromyces sp. FNG5 is tolerant to phenolic monomers and has ability to degrade them. Therefore, such anaerobic fungi may play an important role in fibre degradation in the rumen.