Age‐related cellular and microstructural changes in the rotator cuff enthesis

Rotator cuff injuries increase with age. The enthesis is the most frequent site of rotator cuff injury and degeneration. Understanding age‐related changes of the enthesis are essential to determine the mechanism of rotator cuff injuries, degeneration, and to guide mechanistically driven therapies. I...

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Bibliographic Details
Published inJournal of orthopaedic research Vol. 40; no. 8; pp. 1883 - 1895
Main Authors Long, Zeling, Nakagawa, Koichi, Wang, Zhanwen, Amadio, Peter C., Zhao, Chunfeng, Gingery, Anne
Format Journal Article
LanguageEnglish
Published United States 01.08.2022
Subjects
Adipogenesis
aging
Animals
Chondrogenesis
enthesis
Osteogenesis
Rats
Rotator Cuff
Rotator Cuff Injuries
tenogenesis
enthesis
aging
chondrogenesis
tenogenesis
rotator cuff
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Summary:Rotator cuff injuries increase with age. The enthesis is the most frequent site of rotator cuff injury and degeneration. Understanding age‐related changes of the enthesis are essential to determine the mechanism of rotator cuff injuries, degeneration, and to guide mechanistically driven therapies. In this study, we explored age‐related cellular changes of the rotator cuff enthesis in young, mature, and aged rats. Here we found that the aged enthesis is typified by an increased mineralized zone and decreased nonmineralized zone. Proliferation, migration, and colony‐forming potential of rotator cuff derived cells (RCECs) was attenuated with aging. The tenogenic and chondrogenic potential were significantly reduced, while the osteogenic potential increased in aged RCECs. The adipogenic potential increased in RCECs with age. This study explores the cellular differences found between young, mature, and aged rotator cuff enthesis cells and highlights the importance of using age‐appropriate models, as well as provides a basis for further delineation of mechanisms and potential therapeutics for rotator cuff injuries.
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Author Contribution: ZL, PCA, CZ and AG contributed to conception, study design and data interpretation. ZL, KN, ZW performed experimental assays. ZL, AG completed data analysis. ZL and AG wrote manuscript. All authors contributed to revision of manuscript. All authors have read and approved this manuscript.
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.25211