Increased frequency of LOH on chromosome 9 in sporadic primary melanomas is associated with increased patient age at diagnosis
We carried out statistical analysis of the frequency of loss of heterozygosity (LOH) at 10 microsatellite markers on chromosome 9. In 44 microdissected sporadic primary melanomas a comparison of LOH frequency data with other patient data, like age at diagnosis and tumour thickness, showed an interes...
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Published in | Mutagenesis Vol. 15; no. 3; pp. 257 - 260 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.05.2000
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Subjects | |
Online Access | Get full text |
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Summary: | We carried out statistical analysis of the frequency of loss of heterozygosity (LOH) at 10 microsatellite markers on chromosome 9. In 44 microdissected sporadic primary melanomas a comparison of LOH frequency data with other patient data, like age at diagnosis and tumour thickness, showed an interesting correlation between patient age at diagnosis and frequency of LOH on chromosome 9. The patient group with age >72 years at diagnosis (n = 22, mean age 82.3 ± 6.0 years, mean LOH 3.4 ± 2.3) showed significantly increased LOH frequency (OR 3.1, 95% CI 1.8–5.3; χ2 test, P < 0.0001) compared with age group ≤72 years (n = 22, mean age 56.1 ± 14.5 years, mean LOH 1.8 ± 1.7). A statistically significant increased frequency of LOH (OR 3.5, 95% CI 1.5–7.9; χ2 test, P = 0.03 after Bonferroni correction) was found only at marker D9S736 on 9p22 (telomeric to the INK4–ARF locus) relative to other markers on six different chromosomes. No other marker, including those located within the INK4–ARF locus, showed a statistically significant increased frequency of LOH. Our results for the first time show a non-random tendency for increased allelic loss in melanomas with increased patient age at diagnosis, besides supporting the existence of an additional tumour suppressor gene(s) on chromosome 9. |
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Bibliography: | PII:1464-3804 ark:/67375/HXZ-DZSN5HNG-P local:0150257 istex:9C8473CF0D435EDD8D2782DE598A1CEE19D8A5A4 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0267-8357 1464-3804 1464-3804 |
DOI: | 10.1093/mutage/15.3.257 |