Low-molecular-weight-chitosan ameliorates cadmium-induced toxicity in the freshwater crab, Sinopotamon yangtsekiense

• Published in: Ecotoxicology and environmental safety Vol. 74; no. 5; pp. 1164 - 1170
• Main Authors: Li, Ruijin, Zhou, Yanying, Wang, Lan, Ren, Guorui
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.07.2011; Elsevier
• Subjects: Adenosine Triphosphatases - metabolism; Animal, plant and microbial ecology; Animals; antioxidant activity; Antioxidants; Antioxidants - pharmacology; Applied ecology; Biological and medical sciences; Brachyura - drug effects; Brachyura - metabolism; Ca 2+-ATPase; Ca2-transporting ATPase; Cadmium; Cadmium - metabolism; Cadmium - toxicity; Calcium-Transporting ATPases - metabolism; Chitosan - pharmacology; Control; Crabs; Crustacea; Damage; Decapoda; Ecotoxicology, biological effects of pollution; Fresh Water - chemistry; Freshwater; Freshwaters; Fundamental and applied biological sciences. Psychology; General aspects; Hepatopancreas - drug effects; Hepatopancreas - metabolism; Invertebrates; Kinases; Low-molecular-weight-chitosan; messenger RNA; Metallothionein; Metallothionein - genetics; Metallothionein - metabolism; Na +,K +-ATPase; oxidative stress; Oxidative Stress - drug effects; Protective; protective effect; Protein kinase C; Protein Kinase C - metabolism; Sinopotamon yangtsekiense; sodium-potassium-exchanging ATPase; Sodium-Potassium-Exchanging ATPase - metabolism; toxicity; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Cadmium; Cd; Protein kinase C; GSH-Px; POD; Low-molecular-weight-chitosan; Ca 2+-ATPase; MT; PKC; SOD; LPO; MDA; Na +,K +-ATPase; CaM; LMWC; ANOVA; CAT; ROS; Metallothionein; Sinopotamon yangtsekiense; Toxicity; Fresh water; Biological marker; K; Crustacea; Na; Decapoda; Molecular mass; Ecotoxicology; Enzyme; Transferases; ATPase; Detoxification; Brachyura; Metalloproteins; Heavy metal; Pollutant; Arthropoda; Hydrolases; Environment; exchanging ATPase; Invertebrata; Ca
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2011.02.018

Cadmium (Cd) has been shown to induce oxidative stress. Low-molecular-weight-chitosan (LMWC) has been demonstrated to exhibit potent antioxidant effects. We investigated the regulation role in Cd 2+-induced oxidative damage in the hepatopancreas of the freshwater crab Sinopotamon yangtsekiense and the protective effect of LMWC. The results showed that Cd 2+ significantly increased the hepatopancreatic metallothionein (MT) mRNA levels and protein kinase C (PKC) activity while decreasing the activities of Na +,K +-ATPase and Ca 2+-ATPase in crabs relative to the control group. Co-treatment with LMWC suppressed the levels of MT and PKC but raised the activities of Na +,K+-ATPase and Ca 2+-ATPase in hepatopancreatic tissues compared with the crabs exposed to Cd 2+ alone. We postulate that LMWC may exert its protective effect through regulating the expressions of MT, PKC, Na +,K +-ATPase and Ca 2+-ATPase, thereby enhancing antioxidant defense. These observations suggest that LMWC may be beneficial because of its ability to alleviate the Cd 2+-induced damages to the crabs.