Sulfated glycans containing NeuAcα2-3Gal facilitate the propagation of human H1N1 influenza A viruses in eggs

• Published in: Virology (New York, N.Y.) Vol. 562; pp. 29 - 39
• Main Authors: Ichimiya, Tomomi, Okamatsu, Masatoshi, Kinoshita, Takaaki, Kobayashi, Daiki, Ichii, Osamu, Yamamoto, Naoki, Sakoda, Yoshihiro, Kida, Hiroshi, Kawashima, Hiroto, Yamamoto, Kazuo, Takase-Yoden, Sayaka, Nishihara, Shoko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2021
• Subjects: Allantois - metabolism; amino acids; Amnion - metabolism; Animals; Carbohydrate Sulfotransferases; Chick Embryo - metabolism; Chick Embryo - virology; chickens; Dogs; Egg-adaptation; Galactosides - chemistry; Galactosides - metabolism; Hemagglutinin; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - metabolism; hemagglutinins; human influenza; Humans; Infectious Disease; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - growth & development; Influenza A Virus, H1N1 Subtype - metabolism; Influenza virus; Madin Darby Canine Kidney Cells; Mutation; N-Acetylneuraminic Acid - chemistry; N-Acetylneuraminic Acid - metabolism; pandemic; polysaccharides; Polysaccharides - chemistry; Polysaccharides - metabolism; Receptor; Receptors, Virus - metabolism; Sulfated glycan; Sulfates - chemistry; Sulfates - metabolism; Sulfotransferases - genetics; Sulfotransferases - metabolism; virology; Influenza virus; Receptor; Hemagglutinin; Egg-adaptation; Sulfated glycan
• ISSN: 0042-6822; 1096-0341; 1096-0341
• DOI: 10.1016/j.virol.2021.06.008

When human influenza viruses are isolated and passaged in chicken embryos, variants with amino acid substitutions around the receptor binding site of hemagglutinin (HA) are selected; however, the mechanisms that underlie this phenomenon have yet to be elucidated. Here, we analyzed the receptor structures that contributed to propagation of egg-passaged human H1N1 viruses. The analysis included seasonal and 2009 pandemic strains, both of which have amino acid substitutions of HA found in strains isolated or passaged in eggs. These viruses exhibited high binding to sulfated glycans containing NeuAcα2-3Gal. In MDCK cells overexpressing the sulfotransferase that synthesize Galβ1-4(SO3--6)GlcNAc, production of human H1N1 viruses was increased up to 90-fold. Furthermore, these sulfated glycans were expressed on the allantoic and amniotic membranes of chicken embryos. These results suggest that 6-sulfo sialyl Lewis X and/or NeuAcα2-3Galβ1-4(SO3--6)GlcNAc are involved in efficient propagation of human H1N1 viruses in chicken embryos. •Sulfated glycans are expressed on the allantoic and amniotic membranes of embryonated chicken eggs.•Egg-grown human H1N1 influenza A viruses bind to sulfated glycans containing NeuAcα2-3Gal.•Growth of egg-passaged H1N1 influenza A viruses was increased in MDCK cells overexpressing the sulfated glycans.