Identification of the major selenium compound, Se-Methionine, in three yeast ( Saccharomyces cerevisiae) dietary supplements by on-line narrowbore liquid chromatography–electrospray tandem mass spectrometry

• Published in: Journal of Chromatography A Vol. 1071; no. 1; pp. 191 - 196
• Main Authors: Dumont, Emmie, Cremer, Koen De, Hulle, Marijn Van, Chéry, Cyrille C., Vanhaecke, Frank, Cornelis, Rita
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 15.04.2005; Elsevier
• Subjects: Biological and medical sciences; Chromatography, High Pressure Liquid - methods; dietary mineral supplements; Dietary Supplements - analysis; electrospray tandem mass spectrometry; ES–MS–MS; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; HPLC; liquid chromatography; mass spectrometry; methionine; Methods of analysis, processing and quality control, regulation, standards; Reference Standards; Reproducibility of Results; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Selenium; seleno amino acids; Selenomethionine - analysis; Sensitivity and Specificity; Speciation; Spectrometry, Mass, Electrospray Ionization - methods; Spectrophotometry, Atomic; yeasts; Selenium; Speciation; HPLC; ES–MS–MS; Chemical analysis; Yeast; HPLC chromatography; Selenium Organic compounds; ES-MS-MS; Electrospray; Fungi; Selenium containing aminoacid; Mass spectrometry MS/MS; Ascomycetes; Qualitative analysis; Saccharomyces cerevisiae; Thallophyta; Food supplement; Selenium Compounds
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.11.046

On-line monitoring of six Se-compounds was accomplished by using an XTerra MS C 18 column coupled to electrospray tandem mass spectrometry (ES–MS–MS). In view of the nature of the compounds, the positively charged ion pairing agent tetraethylammoniumchloride (TEACl) was added to the mobile phase. The HPLC–ES–MS–MS method was optimized with six commercially available Se-compounds. Substitution of the analytical column by the narrowbore type significantly enhanced the sensitivity of the method. We were able to detect the m/ z of these six molecules on-line. Furthermore, all product ions could be monitored. The method was applied to three different yeast-based supplements. They were submitted to proteolytic digestion and screened for their Se-content by HPLC–HG–AFS (hydride generation-atomic fluorescence spectrometry). By application of on-line narrowbore HPLC–electrospray tandem mass spectrometry, the main compound present in these three supplements, Se-Methionine, could be measured on its m/ z and its product ions. The method can be further extended for on-line measurement of different Se-species in complex matrices