[Ca2+]i imaging in PC12 cells : multiple response patterns to receptor activation reveal new aspects of transmembrane signaling

Fura-2 imaging microscopy was used to study [Ca2+]i in nerve growth factor-differentiated PC12 cells exposed to agonists (bradykinin, carbamylcholine, and ATP) binding to receptors coupled to polyphosphoinositide hydrolysis. With all the treatments employed, the response to an individual agonist was...

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Published inThe Journal of cell biology Vol. 113; no. 6; pp. 1341 - 1350
Main Authors GROHOVAZ, F, ZACCHETTI, D, CLEMENTI, E, LORENZON, P, MELDOLESI, J, FUMAGALLI, G
Format Journal Article
LanguageEnglish
Published New York, NY Rockefeller University Press 01.06.1991
The Rockefeller University Press
Subjects
Adenosine Triphosphate
Biological and medical sciences
Bradykinin
Calcium - metabolism
Carbachol
Cell Differentiation
Cell Line
Cell Membrane - metabolism
Clone Cells
Fundamental and applied biological sciences. Psychology
Fura-2
General aspects
Image Processing, Computer-Assisted
Microscopy, Fluorescence
Molecular and cellular biology
Nerve Growth Factors
Neurons - metabolism
Receptors, Cell Surface - metabolism
Signal Transduction
Activation
Models
Cell line
Calcium
Biological receptor
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Summary:Fura-2 imaging microscopy was used to study [Ca2+]i in nerve growth factor-differentiated PC12 cells exposed to agonists (bradykinin, carbamylcholine, and ATP) binding to receptors coupled to polyphosphoinositide hydrolysis. With all the treatments employed, the response to an individual agonist was often incomplete, i.e., composed of either release from intracellular stores or influx only. In individual cells the responses were closely similar when only one and the same agonist was employed, and markedly heterogeneous, with considerable variation of the release/influx ratio, when different agonists were delivered in sequence. In a recently isolated PC12 cell clone, heterogeneity of the receptor-induced [Ca2+]i responses was markedly lower than in the overall population, although the release/influx ratio was still variable. We conclude that the large response heterogeneity observed in the overall PC12 cell population is due (a) to the coexistence of multiple clones; and (b) to the variable activation of intracellular transduction mechanisms.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.113.6.1341