Rapid quantification of cyanamide by ultra-high-pressure liquid chromatography in fertilizer, soil or plant samples

• Published in: Journal of Chromatography A Vol. 1216; no. 29; pp. 5614 - 5618
• Main Authors: Nagumo, Yoshifumi, Tanaka, Kazuya, Tewari, Kaushal, Thiraporn, Khwankaew, Tsuchida, Toru, Honma, Toshimitsu, Ohtake, Norikuni, Sueyoshi, Kuni, Takahashi, Yoshihiko, Ohyama, Takuji
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 17.07.2009; Amsterdam; New York: Elsevier; Elsevier
• Subjects: 6-aminoquinolyl- N-hydroxysuccinimidyl-carbamate; agricultural soils; Agronomy. Soil science and plant productions; Biological and medical sciences; calcium cyanamide; chemical constituents of plants; chemical residues; Chromatography, High Pressure Liquid - methods; cover crops; Cyanamide; Cyanamide - analysis; cyanamides; Fertilizer; fertilizer analysis; Fertilizers - analysis; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Generalities. Analysis and diagnosis methods; Hairy vetch; high performance liquid chromatography; Lime nitrogen; nitrogen fertilizers; Plants - chemistry; quantitative analysis; Soil; Soil - analysis; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Ultra-high-pressure liquid chromatography; Vicia villosa; Ultra-high-pressure liquid chromatography; Lime nitrogen; 6-aminoquinolyl- N-hydroxysuccinimidyl-carbamate; Hairy vetch; Soil; Cyanamide; Fertilizer; Ultra performance liquid chromatography; Green manure; Chemical analysis; Derivatization; Diimide; Stem; 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate; Dicotyledones; Angiospermae; Plant growth substance; Quantitative analysis; Below ground plant part; Root; Vicia villosa; Plant leaf; Fertilizers; Soils; Ultraviolet detector; Analysis method; Leguminosae; Plant origin; Spermatophyta; Fodder crop
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2009.05.067

A rapid and simple method for determination of cyanamide in fertilizer, soil and plants has been developed. In this method, cyanamide is extracted with 2% acetic acid and the extract separated by centrifugation. It is then purified by passing through a membrane filter. The extract was derivatized with 6-aminoquinolyl- N-hydroxysuccinimidyl-carbamate and the derivatized compound separated by ultra-high-pressure liquid chromatography. It is then detected with a UV detector at 260 nm by the same method as is used for amino acid analysis. The proposed method is fast, simple and cheap and also has good selectivity and sensitivity for the determination of cyanamide in a wide range of biotic and abiotic materials.