Effect of pH on the bacterial community present in larvae and spat of Crassostrea gigas

• Published in: Latin american journal of aquatic research Vol. 47; no. 3; pp. 513 - 523
• Main Authors: Flores-Higuera, Francisco A, Luis-Villasenor, Irasema E, Rochin-Arenas, Jesus A, Gomez-Gil, Bruno, Mazon-Suastegui, Jose Manuel, Voltolina, Domenico, Medina-Hernandez, Diana
• Format: Journal Article
• Language: English
• Published: Valparaiso Pontificia Universidad Catolica de Valparaiso, Escuela de Ciencias del Mar 01.07.2019; Pontificia Universidad Catolica de Valparaiso; Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
• Subjects: Acidification; Aquaculture; Bacteria; Biodiversity; Bubbling; Carbon dioxide; Communities; Crassostrea gigas; Cultured organisms; Developmental stages; Disease resistance; FISHERIES; Gene sequencing; Larvae; MARINE & FRESHWATER BIOLOGY; Marine environment; Marine molluscs; Next-generation sequencing; Ocean acidification; OCEANOGRAPHY; Oceans; Oysters; pH effects; rRNA 16S; Spat; Water flow; México; ocean acidification; pediveliger larvae; spat; bacterial communities; Crassostrea gigas
• ISSN: 0718-560X; 0718-560X
• DOI: 10.3856/vol47-issue3-fulltext-13

Changes in marine environments, including pH changes, have been correlated to alterations in the physiology and disease susceptibility of cultured organisms at the early stages of development. In this study, high-throughput sequencing of the V3-V4 region of the 16S rRNA gene was performed to evaluate the bacterial biodiversity of Crassostrea gigas pediveliger larvae and spat under acidic stress compared to that of larvae at normal pH value. The evaluation was performed in an experimental system with continuous water flow and pH manipulation by C[O.sub.2] bubbling to simulate acidification (pH 7.38 [+ or -] 0.039), using the current ocean pH conditions (pH 8.116 [+ or -] 0.023) as a reference. The results indicated that the bacterial communities associated with both pediveliger larvae and spat were modified in response to acidic conditions. The families Rhodobacteraceae and Campylobacteraceae were the most affected by the change in pH, with increases in Vibrionaceae in pediveliger larvae and Planctomycetaceae and Phyllobacteriaceae in spat detected. The results of this study demonstrate that the bacterial communities associated with C. gigas pediveliger larvae and spat are responsive to changes in ocean acidification.