The nascent polypeptide-associated complex subunit Egd1 is required for efficient selective mitochondrial degradation in budding yeast

• Published in: Scientific reports Vol. 14; no. 1; p. 546
• Main Authors: Tian, Yuan, Okamoto, Koji
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.01.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/80/39/2348; 631/80/642/333; Autophagy; Autophagy-Related Proteins - genetics; Autophagy-Related Proteins - metabolism; Biodegradation; Homeostasis; Humanities and Social Sciences; Humans; Mitochondria; Mitophagy; multidisciplinary; Null cells; Peptides - metabolism; Phosphorylation; Polypeptides; Receptors, Cytoplasmic and Nuclear - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Saccharomycetales - metabolism; Science; Science (multidisciplinary); Yeasts
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-50245-7

Selective degradation of dysfunctional or excess mitochondria is a fundamental process crucial for cell homeostasis in almost all eukaryotes. This process relies on autophagy, an intracellular self-eating system conserved from yeast to humans and is thus called mitophagy. Detailed mechanisms of mitophagy remain to be fully understood. Here we show that mitochondrial degradation in budding yeast, which requires the pro-mitophagic protein Atg32, is strongly reduced in cells lacking Egd1, a beta subunit of the nascent polypeptide-associated complex acting in cytosolic ribosome attachment and protein targeting to mitochondria. By contrast, loss of the sole alpha subunit Egd2 or the beta subunit paralogue Btt1 led to only a partial or slight reduction in mitophagy. We also found that phosphorylation of Atg32, a crucial step for priming mitophagy, is decreased in the absence of Egd1. Forced Atg32 hyperphosphorylation almost completely restored mitophagy in egd1 -null cells. Together, we propose that Egd1 acts in Atg32 phosphorylation to facilitate mitophagy.