Mechanistic insights into consumption of the food additive xanthan gum by the human gut microbiota

• Published in: Nature microbiology Vol. 7; no. 4; pp. 556 - 569
• Main Authors: Ostrowski, Matthew P., La Rosa, Sabina Leanti, Kunath, Benoit J., Robertson, Andrew, Pereira, Gabriel, Hagen, Live H., Varghese, Neha J., Qiu, Ling, Yao, Tianming, Flint, Gabrielle, Li, James, McDonald, Sean P., Buttner, Duna, Pudlo, Nicholas A., Schnizlein, Matthew K., Young, Vincent B., Brumer, Harry, Schmidt, Thomas M., Terrapon, Nicolas, Lombard, Vincent, Henrissat, Bernard, Hamaker, Bruce, Eloe-Fadrosh, Emiley A., Tripathi, Ashootosh, Pope, Phillip B., Martens, Eric C.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2022; Nature Publishing Group
• Subjects: 38/39; 38/77; 38/91; 45/22; 45/90; 631/326/2565/2134; 631/326/41/2142; 82/58; 82/80; Animal models; Animals; Bacteroides intestinalis; Biomedical and Life Sciences; Dietary Fiber; Digestive system; Digestive tract; Food Additives; Food chains; Food consumption; Food processing; Gastrointestinal Microbiome; Gastrointestinal tract; Germfree; Humans; Infectious Diseases; Intestinal microflora; Life Sciences; Medical Microbiology; Mice; Microbiology; Microbiomes; Microbiota; Oligosaccharides; Parasitology; Polysaccharides; Polysaccharides, Bacterial; Ruminococcaceae; Virology; Xanthan gum
• ISSN: 2058-5276; 2058-5276
• DOI: 10.1038/s41564-022-01093-0

Processed foods often include food additives such as xanthan gum, a complex polysaccharide with unique rheological properties, that has established widespread use as a stabilizer and thickening agent. Xanthan gum’s chemical structure is distinct from those of host and dietary polysaccharides that are more commonly expected to transit the gastrointestinal tract, and little is known about its direct interaction with the gut microbiota, which plays a central role in digestion of other dietary fibre polysaccharides. Here we show that the ability to digest xanthan gum is common in human gut microbiomes from industrialized countries and appears contingent on a single uncultured bacterium in the family Ruminococcaceae. Our data reveal that this primary degrader cleaves the xanthan gum backbone before processing the released oligosaccharides using additional enzymes. Some individuals harbour Bacteroides intestinalis that is incapable of consuming polymeric xanthan gum but grows on oligosaccharide products generated by the Ruminococcaceae. Feeding xanthan gum to germfree mice colonized with a human microbiota containing the uncultured Ruminococcaceae supports the idea that the additive xanthan gum can drive expansion of the primary degrader Ruminococcaceae, along with exogenously introduced B. intestinalis . Our work demonstrates the existence of a potential xanthan gum food chain involving at least two members of different phyla of gut bacteria and provides an initial framework for understanding how widespread consumption of a recently introduced food additive influences human microbiomes. The human gut microbiota can metabolize xanthan gum, a food additive that was introduced to our diets relatively recently in processed foods. A Ruminococcaceae species can degrade this complex polysaccharide and, in some individuals, Bacteroides intestinalis can grow on the released oligosaccharide products.