The Medicago truncatula MtAnn1 gene encoding an annexin is induced by nod factors and during the symbiotic interaction with Rhizobium meliloti

• Published in: Molecular plant-microbe interactions Vol. 11; no. 6; pp. 504 - 513
• Main Authors: Carvalho Niebel, F. de, Lescure, N, Cullimore, J.V, Gamas, P
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.06.1998; American Phytopathological Society; The American Phytopathological Society
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; AMINO ACID SEQUENCES; Annexins - genetics; ARN MENSAJERO; ARN MESSAGER; BINDING PROTEINS; Biological and medical sciences; CALCIO; CALCIUM; CHEMICAL COMPOSITION; CHEMISTRY; CHIMIE; COLONIZATION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Economic plant physiology; EXPRESION GENICA; EXPRESSION DES GENES; FOSFOLIPIDOS; Fundamental and applied biological sciences. Psychology; GENBANK/Y15036; GENE EXPRESSION; GENETIC MARKERS; GENETIC REGULATION; GENETICA; GENETICS; GENETIQUE; HISTOCHEMISTRY; Life Sciences; MARCADORES GENETICOS; MARKER GENES; MARQUEUR GENETIQUE; Medicago sativa - genetics; MEDICAGO TRUNCATULA; MESSENGER RNA; MOLECULAR SEQUENCE DATA; Nitrogen Fixation - genetics; NODOSITE RACINAIRE; NUCLEOTIDE SEQUENCE; NUDOSIDADES RADICULARES; OLIGOSACARIDOS; OLIGOSACCHARIDE; OLIGOSACCHARIDES; PHOSPHATIDE; PHOSPHOLIPIDS; Plant Proteins; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; QUIMICA; RHIZOBIUM MELILOTI; RNA, Messenger - genetics; RNA, Messenger - metabolism; ROOT NODULES; SECUENCIA NUCLEOTIDICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; SIMBIOSIS; Sinorhizobium meliloti - physiology; supernodulating mutant line; SYMBIOSE; SYMBIOSIS; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbiosis; Plant; Rhizobium meliloti; Leguminosae; Dicotyledones; Angiospermae; Vegetal microorganism relation; Bacteria; Medicago; Spermatophyta; Rhizobiaceae; Annexin 1
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI.1998.11.6.504

Here we report the characterization of a new Nod factor induced gene from Medicago truncatula identified by mRNA differential display. This gene, designated MtAnn1, encodes a protein homologous to the annexin family of calcium- and phospholipid-binding proteins. We further show that the MtAnn1 gene is also induced during symbiotic associations with Rhizobium meliloti, both at early stages in bacterial-inoculated roots and in nodule structures. By in situ hybridization, we demonstrate that MtAnn1 expression in nodules is mainly associated with the distal region of invasion zone II not containing infection threads, revealing MtAnn1 as a new marker gene of the pre-infection zone. Moreover, analyses of MtAnn1 expression in response to bacterial symbiotic mutants suggest that the expression of MtAnn1 during nodulation requires biologically active Nod factors and is independent of the infection process