Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer

Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct homogeneous assays that are fast, sensitive, and res...

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Bibliographic Details
Published inJournal of biomolecular screening Vol. 5; no. 5; pp. 297 - 306
Main Author Owicki, J C
Format Journal Article
LanguageEnglish
Published United States 01.10.2000
Subjects
Binding, Competitive
Drug Evaluation, Preclinical - instrumentation
Drug Evaluation, Preclinical - methods
Fluorescence
Fluorescence Polarization - instrumentation
Fluorescence Polarization - methods
Ligands
Receptors, Cell Surface - metabolism
Research Design
Sensitivity and Specificity
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Summary:Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct homogeneous assays that are fast, sensitive, and resistant to some significant interferences. Moreover, the assays are relatively inexpensive. This review surveys the peer-reviewed literature on the subject and explores some of the fundamental issues that bear on assay performance.
ISSN:1087-0571
2472-5552
DOI:10.1177/108705710000500501