In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

• Published in: Acta pharmaceutica (Zagreb, Croatia) Vol. 62; no. 3; pp. 371 - 382
• Main Authors: Kamdem, Jean Paul, Stefanello, Sílvio Terra, Boligon, Aline Augusti, Wagner, Caroline, Kade, Ige Joseph, Pereira, Romaiana Picada, Preste, Alessandro De Souza, Roos, Daniel Henrique, Waczuk, Emily Pansera, Appel, Andre Storti, Athayde, Margareth Linde, Souza, Diogo Onofre, Rocha, João Batista Teixeira
• Format: Journal Article
• Language: English
• Published: Croatia Versita 01.09.2012; De Gruyter Poland
• Subjects: Animals; antioksidans; antioxidant; Antioxidants - analysis; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Brain - drug effects; Brain - metabolism; Brazil; Ethnopharmacology; fenoli; flavonoidi; flavonoids; Flavonoids - analysis; Flavonoids - pharmacology; Ključne riječi: Trichilia catigua (Meliaceae); Lipid Peroxidation - drug effects; Male; Meliaceae - chemistry; Membrane Potential, Mitochondrial - drug effects; Mitochondria, Liver - drug effects; Mitochondria, Liver - metabolism; oksidativni stres; Osmolar Concentration; oxidative stress; phenolics; Phenols - analysis; Phenols - pharmacology; Plant Bark - chemistry; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Stems - chemistry; Rats; Rats, Wistar; reactive oxygen species; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; reaktivne kisikove specije; Solvents - chemistry; Trichilia catigua (Meliaceae); Brazil
• ISSN: 1330-0075; 1846-9558
• DOI: 10.2478/v10007-012-0026-x

Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL-1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g-1 of dry extract. Fe2+-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca2+-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Dym only at high concentrations (40-100 mg mL-1), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties. U radu je opisano ispitivanje antioksidativnog u~inka etanolnog ekstrakta i pojedinih frakcija kore stabljike T. catigua. IC50 (za DPPH test) varirao je izme|u 9,17 ± 0,63 i 76,42 ± 5,87 mg mL-1, a ukupni sadr`aj fenola od 345,63 ± 41,08 i 601,27 ± 42,59 mg GAE po gramu suhog ekstrakta. Etanolni ekstrakt i frakcije zna~ajno su reducirale Fe2+-induciranu lipidnu peroksidaciju. Nadalje, reducirana je oksidacija diklorfluoresceina inducirana ionima kalcija u mitohondrijima, a redukcija je ovisila o dozi etanolnog ekstrakta. Etanolni ekstrakt smanjio je mitohondrijsku Dym samo pri visokim koncentracijama (40 ± 100 mg mL-1), {to ukazuje da se toksi~nost ne preklapa s antioksidativnim u~inkom. Rezultati pokazuju da u farmakolo{ko djelovanje T. catigua treba uklju~iti i antioksidativni u~inak.