Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities

• Published in: Food science and human wellness Vol. 7; no. 3; pp. 209 - 214
• Main Authors: Long, Xingyao, Pan, Yanni, Zhao, Xin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2018; Tsinghua University Press
• Subjects: Antioxidant; Colitis; Kudingcha polyphenols; mRNA expression; Oxazolone; Oxazolone; Kudingcha polyphenols; Colitis; Antioxidant; mRNA expression
• ISSN: 2213-4530; 2213-4530
• DOI: 10.1016/j.fshw.2018.06.002

This research the preventive effect of Kudingcha polyphenols (Kp) on colitis based on animal experiments. Experimental mice divided into four groups, including normal group, model group, low-concentration Kp (LKP) group and high-concentration Kp (HKP) group, and they all smeared and given 0.15mL 1% oxazolone solution by lavage to induce BALB/c mice colitis. DAI, colon weight/length ratio, serum levels of cytokines, related antioxidant activities of colon tissues such, and the mRNA expressions. The experimental results show that KP can significantly (p<0.05) reduce DAI in mice with colitis and inhibit shortening of colon caused by colitis, increase colon weight/length ratio. It can also significantly reduce (p<0.05) the activities of MPO, NO, MDA in mice colon tissues with colitis, increase the activity of GSH, and significantly increase (p<0.05) the level of IL-2 and decrease the level of IL-10 in mice serum with colitis. The results of qPCR assay show that Kp can significantly increase (p<0.05) the expression of Cu/Zn-SOD, Mn-SOD, GSH-Px, CAT, c-Kit, SCF and decrease the expression of IL-8, CXCR2 in mice colon tissues with colitis. Kp has a good preventive effect on colitis, and high concentration of PT has better preventive effect.