Nucleotide sequence of the gene encoding the 45-kilodalton subunit of alcohol dehydrogenase from Acetobacter aceti

• Published in: Journal of fermentation and bioengineering Vol. 73; no. 6; pp. 419 - 424
• Main Authors: Inoue, Takafumi, Sunagawa, Megumi, Mori, Akihiko, Imai, Chuhei, Fukuda, Masao, Takagi, Masamichi, Yano, Keiji
• Format: Journal Article
• Language: English
• Published: Osaka Elsevier B.V 01.01.1992; Society for Fermentation and Bioengineering
• Subjects: ACETOBACTER; Acetobacter aceti; ALCOHOL DEHYDROGENASE; ALCOHOL DESHIDROGENASA; ALCOOL DESHYDROGENASE; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes. Genome; Molecular and cellular biology; Molecular genetics; NUCLEOTIDE SEQUENCE; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Cytochrome c; Nucleotide sequence; Enzyme; Sequence alignment; Bacteria; Homology; Acetobacter aceti; Alcohol dehydrogenase; Subunit; Comparative study; Aminoacid sequence
• ISSN: 0922-338X
• DOI: 10.1016/0922-338X(92)90130-M

The gene encoding the 45 kilodalton subunit of alcohol dehydrogenase from Acetobacter aceti was found to exist in the 3′ flanking region of adhA which encodes the dehydrogenase subunit of the alcohol dehydrogenase. The amino acid sequence of the 45-kilodalton subunit, which was deduced from the DNA sequence, seemed to consist of three mono-heme cytochrome c polypeptide chains joined to form a single tri-heme cytochrome c. The cytochrome c-552 from Thermus aquaticus, which had been reported to be a unique mono-heme cytochrome c, and the 45-kilodalton subunit were found to have considerable similarity in their amino acid sequences.