Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

• Published in: Journal of Zhejiang University. B. Science Vol. 14; no. 2; pp. 97 - 105
• Main Authors: Feng, Bing, Ma, Lai-ji, Yao, Jin-jing, Fang, Yun, Mei, Yan-ai, Wei, Shao-min
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Zhejiang University Press 01.02.2013; Springer Nature B.V; Zhejiang University Press
• Subjects: Avena - chemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cells, Cultured; Child; Cytoprotection; Fibroblasts - drug effects; Fibroblasts - pathology; Fibroblasts - physiology; Humans; Hydrogen Peroxide - pharmacology; Male; Plant Extracts - pharmacology; Seeds - chemistry; Skin Physiological Phenomena - drug effects; 形态学; 生理学; 细胞形成; 细胞生物学; Human dermal fibroblasts; Extraction; Cell injury; Antioxidant; Q28; Oat bran
• ISSN: 1673-1581; 1862-1783; 1862-1783
• DOI: 10.1631/jzus.B1200159

Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investi- gation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide （H2O2）. Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography （HPLC） analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical scavenging activity indicate that oat peptide-dch extract has a direct and concentration-dependent antioxidant activity. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling （TUNEL） assay for apoptosis showed that administration of H2O2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H2O2, but ap- plication oat peptides with H2O2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H2O2-induced decrease of superoxide dismutase （SOD） and the inhibition of malondialdehyde （MDA）. The results demonstrate that oat peptides possess antioxidant activity and are effective against H2O2-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury.