Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro

• Published in: International immunopharmacology Vol. 59; pp. 47 - 52
• Main Authors: Li, Shi-fang, Shao, Jun-jun, Zhao, Fu-rong, Gong, Mei-jiao, Xie, Yin-li, Chang, Hui-yun, Zhang, Yong-guang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2018; Elsevier BV
• Subjects: Adaptive immunity; Antiviral activity; Antiviral agents; E coli; FMDV; Foot & mouth disease; Gene expression; Genes; Immune response; Immune system; Infections; Interferon; Livestock; Myxovirus resistance proteins; Porcine interferon-delta; Serotypes; Swine; Type I interferon; Vaccines; Viruses; Porcine interferon-delta; FMDV; Type I interferon; Antiviral activity
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2018.03.022

Foot-and-mouth disease (FMD) is one of the most devastating diseases affecting livestock. Since vaccines fail to provide protection until seven days post-vaccination, the application of anti-viral molecules is imperative for suppressing the spread of FMDV prior to development of an adaptive immune response. Interferons (IFNs) are effective for the host to fight FMDV infections; however, a novel type I IFNs, interferon delta (IFN-δ), has not been investigated for their antiviral effects against this virus. Thus, this study investigated FMDV infection, upon pre- and post-treatment with PoIFN-δ8. Real-time quantitative PCR was used to quantify the expression levels of IFN-stimulated genes (ISGs), including ISG15, OAS1, PKR, and Mx1. Results showed the PoIFN-δ8 lacking its signal sequence was efficiently expressed in Escherichia coli, and the purified recombinant PoIFN-δ8 exerted a significantly protective effect against two different serotypes of FMDV in IBRS-2 cells. In addition, PoIFN-δ8 induced the expression of IFN-stimulated genes. These findings highlight the significance of PoIFN-δ might serve as an antiviral agent for the prevention of FMDV in pigs and will stimulate the study of exploiting the potential biological functions of IFN-δ in the future. •This is the first study to demonstrate the antiviral activities of IFN-δ against FMDV using IFN-δ8 in vitro.•IFN-δ8 could exert a significantly protective effect against two different serotypes of FMDV in IBRS-2 cells.•PoIFN-δ8 induced expression of Mx1, ISG15, PKR and OAS1.