Protein turnover modifications induced by the protein feeding pattern still persist after the end of the diets

• Published in: American journal of physiology: endocrinology and metabolism Vol. 278; no. 5; pp. E902 - E909
• Main Authors: Arnal, M. A, Mosoni, L, Boirie, Y, Gachon, P, Genest, M, Bayle, G, Grizard, J, Arnal, M, Antoine, J. M, Beaufrere, B, Mirand, P. Patureau
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.05.2000
• Subjects: Adaptation, Physiological; Adult; Aged; Aging; Bicarbonates; Blood Glucose - analysis; Carbon Isotopes; Deuterium; Diet; Dietary Proteins - administration & dosage; Endocrinology and metabolism; Energy Intake; Female; Food; Human health and pathology; Humans; Insulin - blood; Kinetics; Leucine; Life Sciences; Proteins - metabolism
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/ajpendo.2000.278.5.E902

1  Unité d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique et Centre de Recherche en Nutrition Humaine, 63122 Clermont-Ferrand-Theix; 2  Laboratoire de Nutrition Humaine, Université d'Auvergne et Centre de Recherche en Nutrition Humaine, 63009 Clermont-Ferrand; and 3  Société Danone, 92350 Le Plessis-Robinson, France. This study was undertaken to determine whether the protein feeding pattern could induce chronic adaptation of protein turnover. After a 15-day adaptive period, elderly (68 yr) and young (26   yr) women received, for 14 days, a diet providing 200 KJ · kg fat-free mass (FFM) 1 · day 1 , where the daily protein intake (1.7 g protein · kg FFM 1 · day 1 ) was either spread over 4 meals in the spread pattern or mainly (80%) consumed at noon in the pulse pattern. One day after the end of the dietary treatment, whole body leucine kinetics were measured by use of a continuous [ 13 C]leucine infusion, both in the postabsorptive state and in the same fed state. The pulse pattern was able to induce, in young as in elderly women, a lower postabsorptive leucine oxidation and endogenous leucine flux than the spread pattern and improved the responsiveness of nonoxidative leucine disposal during 4-h oral feeding. Thus the pulse pattern was able to induce chronic regulation of protein metabolism in young as in elderly women. women; postabsorptive state; postprandial state; aging