Separation efficiencies in hydrophilic interaction chromatography

• Published in: Journal of Chromatography A Vol. 1184; no. 1; pp. 474 - 503
• Main Authors: Ikegami, Tohru, Tomomatsu, Kouki, Takubo, Hirotaka, Horie, Kanta, Tanaka, Nobuo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.03.2008; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Acetates - isolation & purification; Amide-silica; Amino-silica; Analytical chemistry; Bare silica; CD-silica; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Diol-silica; Exact sciences and technology; Flavonols - isolation & purification; HILIC; Oligosaccharides - isolation & purification; Other chromatographic methods; Poly(succinimide)-silica; Polymer monolithic columns; Purines - isolation & purification; Pyrimidines - isolation & purification; Separation efficiency; Silica monolithic column; Silicon Dioxide - chemistry; Spectrometry, Mass, Electrospray Ionization; Sulfoalkylbetaine-silica; Temperature; Tetracyclines - isolation & purification; Poly(succinimide)-silica; Sulfoalkylbetaine-silica; Polymer monolithic columns; Amino-silica; CD-silica; Silica monolithic column; Separation efficiency; Diol-silica; Bare silica; Amide-silica; HILIC; Polyimide; Peak resolution; Review; Plate efficiency; Silica gel; Modified material; Stationary phase; Hydrophilic Interaction Liquid chromatography; Monolithic column; Liquid chromatography; Cyclodextrin; Application; Betaines; Bonded stationary phase
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2008.01.075

Hydrophilic interaction chromatography (HILIC) is important for the separation of highly polar substances including biologically active compounds, such as pharmaceutical drugs, neurotransmitters, nucleosides, nucleotides, amino acids, peptides, proteins, oligosaccharides, carbohydrates, etc. In the HILIC mode separation, aqueous organic solvents are used as mobile phases on more polar stationary phases that consist of bare silica, and silica phases modified with amino, amide, zwitterionic functional group, polyols including saccharides and other polar groups. This review discusses the column efficiency of HILIC materials in relation to solute and stationary phase structures, as well as comparisons between particle-packed and monolithic columns. In addition, a literature review consisting of 2006–2007 data is included, as a follow up to the excellent review by Hemström and Irgum.