Expression of genes encoding laccase and manganese-dependent peroxidase in the fungus Ceriporiopsis subvermispora is mediated by an ACE1-like copper-fist transcription factor

• Published in: Fungal genetics and biology Vol. 46; no. 1; pp. 104 - 111
• Main Authors: Álvarez, José Miguel, Canessa, Paulo, Mancilla, Rodrigo A., Polanco, Rubén, Santibáñez, Paulina A., Vicuña, Rafael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2009
• Subjects: ACE; ACE1-like copper-fist transcription factor; Amino Acid Sequence; Basidiomycetes; biodegradation; Ceriporiopsis subvermispora; chemistry; Copper; Copper - pharmacology; copper-fist; Coriolaceae; Coriolaceae - enzymology; DNA, Fungal; DNA, Fungal - metabolism; drug effects; Electrophoretic mobility-shift assays; enzyme activity; enzymology; fungal proteins; fungus physiology; gene expression; Gene Expression Regulation, Fungal; Gene Expression Regulation, Fungal - drug effects; genes; genetic complementation; Genetic Complementation Test; genetics; Laccase; Laccase - genetics; Laccase - metabolism; lignin; Ligninolytic; Manganese peroxidase; metabolism; microbial genetics; Molecular Sequence Data; Multicopper oxidase; peptidyl-dipeptidase A; Peroxidases; Peroxidases - metabolism; Phanerochaete chrysosporium; pharmacology; physiological regulation; Promoter Regions, Genetic; Promoter Regions, Genetic - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; transcription (genetics); Transcription factor; transcription factors; Transcription Factors - chemistry; Transcription Factors - metabolism; transcriptional activation; white-rot fungi; Multicopper oxidase; Ceriporiopsis subvermispora; ACE; Manganese peroxidase; EMSA; Ligninolytic; MCO; Electrophoretic mobility-shift assays; Transcription factor; Copper; copper-fist; Laccase
• ISSN: 1087-1845; 1096-0937; 1096-0937
• DOI: 10.1016/j.fgb.2008.10.002

The effect of copper on the expression of genes encoding the ligninolytic enzymes laccase ( lcs) and manganese peroxidase ( mnp) in Ceriporiopsis subvermispora was evaluated. This metal increased transcript levels of lcs, mnp1 and mnp2. This finding was not unexpected in the case of lcs, since its promoter contains a putative ACE element. Originally characterized in the yeast Saccharomyces cerevisiae, ACE is the target sequence of the ACE1 copper-responsive transcription factor in this microorganism. Analysis of the promoter regions of mnp genes revealed the presence of formerly unnoticed ACE elements. Based on the ace1 gene from Phanerochaete chrysosporium, we isolated and characterized an ACE1-like transcription factor from C. subvermispora (Cs-ACE1) through complementation of a S. cerevisiae ace1Δ strain. Surprisingly, ACE1 factors from both basidiomycetes exhibit substantial differences, not only structurally but also in their ability to complement the aforementioned yeast strain. Specific binding of Cs-ACE1 to its cognate DNA sequence was confirmed by electrophoretic mobility-shift assays.