Mixed-function oxidase-specific activity in wild and caged speckled sanddabs Citharichthys stigmaeus in Elkhorn Slough, Moss Landing Harbor and nearshore Monterey Bay, California

• Published in: Environmental pollution (1987) Vol. 84; no. 2; pp. 179 - 188
• Main Authors: Rice, David W., Seltenrich, Craig P., Keller, Marilyn L., Spies, Robert B., Felton, James S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1994; Elsevier
• Subjects: Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Citharichthys stigmaeus; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; hydrocarbons; Marine; Marine and brackish environment; marine sediments; mixed-function oxidase sanddabs; pollutants; Pacific Ocean; California; United States; North America; East Pacific; America; INE, USA, California, Monterey Bay; USA, California; mixed-function oxidase sanddabs; hydrocarbons; marine sediments; pollutants; Marine environment; Vertebrata; Enzymatic activity; Hydrocarbon; Enzyme; Pisces; Liver; Mixed function enzyme; Oxidase; Biological indicator; Water pollution; Biological monitoring
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/0269-7491(94)90101-5

The goal of this study was to characterize the spatial, seasonal and annual hepatic activities of mixed-function oxidase (MFO) in the speckled sanddab Citharichthys stigmaeus, the most common fish in the Moss Landing area. In addition, techniques to monitor MFO activities in caged speckled sanddabs were developed and tested. Once the relationship between MFO activities in caged and wild fish populations is determined, caged fish could be used to monitor potential hydrocarbon impacts at Moss Landing, or other marine sites. During each of the spatial, seasonal and annual sediment samplings conducted in 1985–1987 as part of a separate hydrocarbon variability study at Moss Landing, 12 wild speckled sanddabs were collected from Moss Landing Harbor, Elkhorn Slough and nearshore Monterey Bay sites. In addition, four locations were chosen for a 14-day field caged fish experiment. The caged fish experiments successfully demonstrated the feasibility of using caged sanddabs as indicators of hydrocarbon exposure. The major source of variability in hepatic aryl hydrocarbon hydroxylase activity in wild speckled sanddabs from the Moss Landing area is due to seasonal rather than site differences. Significant relationships between caged fish MFO response and sediment hydrocarbon concentrations were found. It is possible that caged fish could be used in place of costly sediment sampling and analysis, and provide a more direct method to assess biological impacts. Many of the caging techniques demonstrated in this study can easily be transferred to other benthic flatfish, and other marine and freshwater environments.