occurrence of 4-methylthiazole-5-acetic acid as a thiamine metabolite in rabbit, dog, man and rat

• Published in: Journal of biochemistry (Tokyo) Vol. 65; no. 6; pp. 953 - 960
• Main Authors: Matsuo, T, Suzuoki, Z
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.06.1969
• Subjects: Acetates - urine; Animals; Biological Sciences; Carbon Isotopes; Chromatography, Ion Exchange; Crystallization; Dogs; Female; Humans; Male; Rabbits; Radioisotope Dilution Technique; Rats; Thiamine - administration & dosage; Thiamine - metabolism; Thiamine - urine; Thiazoles - urine; Time Factors
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a129100

The urinary excretion of 4-methylthiazole-5-acetic acid (MTA) was investigated on rabbits, dog, human subjects and rats receiving orally thiamine or thiamine tetrahydro-furfuryl disulfide (TTFD). The qualitative and quantitative determinations of MTA were performed by either direct or invert isotope dilution method following chromato-graphic separation using Amberlite CG-50 ion exchanger. In rabbits MTA accounted for 14 to 57% of radioactivity excreted into the 24 hr urine and it corresponded to 4 to 35% of the administered 14C-thiamine. In a dog 6.4% of the administered thiamine was characterized as MTA in the 1–6hr urine. The occurrence of MTA was also confirmed in the human urine by the direct isotope dilution method. The average amount of MTA excreted for 3 hr was 1.38 mg in untreated subjects and was increased about 2- or 3-fold after ingestion of thiamine (500 mg) or TTFD. From these results it is concluded that MTA is a urinary metabolite of orally administered thiamine in the mammalians so far examined.