Immunohistochemical evidence for the localization of neurons containing the putative transmitter l-proline in rat brain

We examined whether there are the neurotransmitter candidate amino acid l-proline containing neurons localized in the rat brain. Antibodies against l-proline conjugated with rabbit serum albumin were raised in a rabbit and purified with affinity chromatography. Strong l-proline-like immunoreactivity...

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Bibliographic Details
Published inBrain research Vol. 1073; pp. 311 - 315
Main Authors Takemoto, Yumi, Semba, Reiji
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 16.02.2006
Subjects
Animals
Brain - cytology
Brainstem
Hypothalamus
Immunoblotting - methods
Immunohistochemistry
Immunohistochemistry - methods
l-proline
Male
Neurons - metabolism
Proline - metabolism
Putative neurotransmitter
Rat
Rats
Rats, Wistar
Immunohistochemistry
Putative neurotransmitter
Hypothalamus
l-proline
Rat
Brainstem
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Summary:We examined whether there are the neurotransmitter candidate amino acid l-proline containing neurons localized in the rat brain. Antibodies against l-proline conjugated with rabbit serum albumin were raised in a rabbit and purified with affinity chromatography. Strong l-proline-like immunoreactivity was confined to several groups of neurons in the arcuate nucleus (n) and supraoptic n in the hypothalamus and area postrema. The brainstem had markedly stained fibers in the medial longitudinal fasciculus and localized neuronal cell body labeling in the red n, mesencephalic trigeminal n, lateral reticular n, raphe obscurus n, solitary n, compact ambiguus n, motor trigeminal n and n of trapezoid body. Our findings are consistent with the hypothesis that l-proline may function as a neurotransmitter or neuromodulator in the brain.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2005.12.064