HTS-Corrector: software for the statistical analysis and correction of experimental high-throughput screening data

• Published in: Bioinformatics Vol. 22; no. 11; pp. 1408 - 1409
• Main Authors: Makarenkov, Vladimir, Kevorkov, Dmytro, Zentilli, Pablo, Gagarin, Andrei, Malo, Nathalie, Nadon, Robert
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.2006; Oxford Publishing Limited (England)
• Subjects: Algorithms; Biological and medical sciences; Computational Biology - methods; Computer Simulation; Computers; Drug Design; Drug Evaluation, Preclinical; Drug Industry; Fundamental and applied biological sciences. Psychology; General aspects; Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects); Models, Statistical; Quality Control; Reproducibility of Results; Software; Technology, Pharmaceutical; High throughput screening; Drug; Visualization; Statistical analysis; Experimental data; Standardization; Error; Minimization; Software; Bioinformatics; Scope note; Corrections
• ISSN: 1367-4803; 1460-2059; 1367-4811
• DOI: 10.1093/bioinformatics/btl126

Motivation: High-throughput screening (HTS) plays a central role in modern drug discovery, allowing for testing of >100 000 compounds per screen. The aim of our work was to develop and implement methods for minimizing the impact of systematic error in the analysis of HTS data. To the best of our knowledge, two new data correction methods included in HTS-Corrector are not available in any existing commercial software or freeware. Results: This paper describes HTS-Corrector, a software application for the analysis of HTS data, detection and visualization of systematic error, and corresponding correction of HTS signals. Three new methods for the statistical analysis and correction of raw HTS data are included in HTS-Corrector: background evaluation, well correction and hit-sigma distribution procedures intended to minimize the impact of systematic errors. We discuss the main features of HTS-Corrector and demonstrate the benefits of the algorithms. Availability: The Microsoft Windows version and a detailed description of the software are freely available at the following URL: Contact:makarenkov.vladimir@uqam.ca