Investigating Vα7.2+/CD161− T Cell and MAIT Cell Profiles Using Flow Cytometry in Healthy Subjects and Subjects with Atopic Dermatitis

• Published in: International journal of molecular sciences Vol. 25; no. 6; p. 3486
• Main Authors: Singh, Parvind, Gaspar, Krisztian, Szegedi, Andrea, Sajtos, Laszlo, Barath, Sandor, Hevessy, Zsuzsanna
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.03.2024; MDPI
• Subjects: Antigens; Atopic dermatitis; B cells; Dendritic cells; Dermatitis; Dermatitis, Atopic; Development and progression; Disease; Ethylenediaminetetraacetic acid; Flow Cytometry; Healthy Volunteers; Humans; Inflammation; Investigations; Lymphocytes; Medical equipment and supplies industry; Medical test kit industry; Mucosal-Associated Invariant T Cells; Pathogenesis; Skin; T cell receptors; T cells; Tumor Necrosis Factor-alpha; Tumor necrosis factor-TNF; United States; atopic dermatitis; dimensionality reduction; flow cytometry; unsupervised clustering; Vα7.2+/CD161− T cells; MAIT cells
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25063486

This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161− T cells in peripheral blood samples from 14 atopic dermatitis patients and 10 healthy controls. Flow cytometry and machine learning algorithms were employed for a comprehensive analysis. The results indicate a significant decrease in MAIT cells and CD69 subsets in atopic dermatitis, coupled with elevated CD38 and polyfunctional MAIT cells producing TNFα and Granzyme B (TNFα+/GzB+). Vα7.2+/CD161− T cells in atopic dermatitis exhibited a decrease in CD8 and IFNγ-producing subsets but an increase in CD38 activated and IL-22-producing subsets. These results highlight the distinctive features of MAIT cells and Vα7.2+/CD161− T cells and their different roles in the pathogenesis of atopic dermatitis and provide insights into their potential roles in immune-mediated skin diseases.