Rapid Fluorescence Spectroscopic Characterization of Salivary DNA of Normal Subjects and OSCC Patients Using Ethidium Bromide

• Published in: Journal of fluorescence Vol. 25; no. 1; pp. 79 - 85
• Main Authors: Yuvaraj, Manoharan, Aruna, Prakasarao, Koteeswaran, Dornadula, Tamilkumar, Palraj, Ganesan, Singaravelu
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.01.2015
• Subjects: Adult; Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Bromides; Carcinoma, Squamous Cell; Deoxyribonucleic acid; DNA - analysis; DNA - chemistry; Ethidium - chemistry; Excitation; Female; Fluorescence; Humans; Kinetics; Male; Middle Aged; Mouth Neoplasms; Original Paper; Patients; Saliva; Saliva - chemistry; Spectrometry, Fluorescence - methods; Statistical analysis; Steady state; Salivary DNA; Ethidium bromide; Saliva mass screening; Simple and rapid technique; Fluorescence steady and excited state kinetics

Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited s...