Rapid Fluorescence Spectroscopic Characterization of Salivary DNA of Normal Subjects and OSCC Patients Using Ethidium Bromide

• Published in: Journal of fluorescence Vol. 25; no. 1; pp. 79 - 85
• Main Authors: Yuvaraj, Manoharan, Aruna, Prakasarao, Koteeswaran, Dornadula, Tamilkumar, Palraj, Ganesan, Singaravelu
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.01.2015
• Subjects: Adult; Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Bromides; Carcinoma, Squamous Cell; Deoxyribonucleic acid; DNA - analysis; DNA - chemistry; Ethidium - chemistry; Excitation; Female; Fluorescence; Humans; Kinetics; Male; Middle Aged; Mouth Neoplasms; Original Paper; Patients; Saliva; Saliva - chemistry; Spectrometry, Fluorescence - methods; Statistical analysis; Steady state; Salivary DNA; Ethidium bromide; Saliva mass screening; Simple and rapid technique; Fluorescence steady and excited state kinetics
• ISSN: 1053-0509; 1573-4994; 1573-4994
• DOI: 10.1007/s10895-014-1482-0

Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited state kinetics of salivary DNA has been performed with 27 normal subjects and 67 OSCC patients saliva using ethidium bromide as a probe to look for the possibility in discrimination between them. On statistical analysis the sensitivity and specificity of 88.9 and 94.0 % has been achieved from the fluorescence emission spectra and 88.9 and 92.5 % with that of fluorescence excitation.