Complete amino acid sequence of human placental 17β-hydroxysteroid dehydrogenase deduced from cDNA

• Published in: FEBS letters Vol. 239; no. 1; pp. 73 - 77
• Main Authors: Peltoketo, H., Isomaa, V., Mäentausta, O., Vihko, R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.10.1988; Elsevier
• Subjects: 17-Hydroxysteroid dehydrogenase; 17-Hydroxysteroid Dehydrogenases - genetics; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA - genetics; Enzymes and enzyme inhibitors; Estrogen; Female; Fundamental and applied biological sciences. Psychology; Humans; Hydrolases; Molecular cloning; Molecular Sequence Data; Nucleotide sequence; Placenta - enzymology; Pregnancy; Protein Conformation; Restriction Mapping; Amino acid sequence; Nucleotide sequence; Molecular cloning; 17-Hydroxysteroid dehydrogenase; Estrogen; Human; Sequencer; Placenta; Complementary DNA; Radiolabelling; Enzyme; Hydrophobicity; 20α-Hydroxysteroid dehydrogenase; Aminoacid sequence; Immunological method
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(88)80548-9

CDNA clones for 17β-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) were isolated from a placental λgt11 expression library using polyclonal antibodies against placental 17-HSD. The largest cDNA contained 1325 nucleotides, consisting of a short 5′-noncoding segment, a coding segment of 987 nucleotides terminated by a TAA codon, and a 329 nucleotide long 3′-noncoding segment. The open reading frame encoded a polypeptide of 327 amino acid residues with a predicted M r of 34853. The amino acid sequence of 23 N-terminal amino acids determined from purified 17-HSD agreed with the sequence deduced from cDNA. The deduced amino acid sequence also contained two peptides previously characterized from the proposed catalytic area of placental 17-HSD.