Atrial natriuretic factor receptors and stimulation of cyclic GMP formation in normal and malignant osteoblasts

• Published in: FEBS letters Vol. 208; no. 2; pp. 263 - 268
• Main Authors: Fletcher, A.E., Allan, E.H., Casley, D.J., Martin, T.J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.11.1986; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Atrial Natriuretic Factor - metabolism; Atrial natriuretic factor cyclic GMP Osteoblast; Atrial natriuretic factorcyclic GMPOsteoblast; atrial natriuretic peptide; Biological and medical sciences; Calcitonin - pharmacology; Calcitonin Gene-Related Peptide; Cyclic AMP - biosynthesis; cyclic GMP; Cyclic GMP - biosynthesis; Fundamental and applied biological sciences. Psychology; Guanylate Cyclase - metabolism; Neuropeptides - pharmacology; osteoblasts; Osteoblasts - metabolism; Parathyroid Hormone - pharmacology; Proteins; Rats; Receptors, Atrial Natriuretic Factor; Receptors, Cell Surface - metabolism; Atrial natriuretic factor cyclic GMP Osteoblast; Cell culture; Vertebrata; Mammalia; Natriuretic hormone; Rat; Rodentia; Peptide hormone; Production; Osteoblast; 3',5'-GMP
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)81029-8

Synthetic rat atrial natriuretic factor (Ile-ANF-26) stimulated cyclic GMP formation by up to several hundred-fold in osteoblast-rich cultures from newborn rat calvaria and in clonal osteogenic sarcoma cells (UMR 106-01) which are phenotypically osteoblast. ANF had no effect on the cyclic AMP response to parathyroid hormone in the same cells. Specific, high-affinity binding sites for ANF were identified in both cell types, with K d and receptor numbers in normal osteoblasts of 1.2 ± 0.1 × 10 −10 M and 42 ± 4 × 10 3 per cell, and in UMR 106-01 cells of 1.4 ± 0.1 × 10 −10M and 22 ± 4 × 10 3per cell.