Cellular fibronectin is induced by epidermal growth factor, but not by dexamethasone or cyclic AMP in rat liver epithelial cells

• Published in: FEBS letters Vol. 239; no. 1; pp. 113 - 116
• Main Authors: Seebacher, Thomas, Manske, Marianne, Kornblihtt, Alberto R., Bade, Ernesto G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.10.1988; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cell migration; Cells, Cultured; Cellular fibronectin; Cyclic AMP - pharmacology; Dexamethasone - pharmacology; Epidermal growth factor; Epidermal Growth Factor - genetics; Epidermal Growth Factor - pharmacology; Epithelium - drug effects; Epithelium - metabolism; Extracellular matrix; Fibronectins - biosynthesis; Fundamental and applied biological sciences. Psychology; Gene expression; Glycoproteins; Insulin - pharmacology; Liver - drug effects; Liver - metabolism; mRNA splicing variant; Proteins; Rat liver epithelial cell; Rats; Reference Values; RNA Splicing; RNA, Messenger - drug effects; RNA, Messenger - genetics; Epidermal growth factor; mRNA splicing variant; Extracellular matrix; Cellular fibronectin; Gene expression; Cell migration; Rat liver epithelial cell; Cell culture; Dexamethasone; Radiolabelling; Rat; Gel electrophoresis; Induction; Liver; Rodentia; Cyclic AMP; Glycoproteins; Fibronectin; Proteins; Vertebrata; Mammalia; Messenger RNA; Immunoblotting assay; Epithelial cell
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(88)80556-8

Epidermal growth factor (EGF) induces fibronectin (FN) and FN mRNA in rat liver epithelial cells, under conditions where the factor also induces the cells to migrate. Newly synthesized protein is secreted into the medium and deposited as substratum-bound extracellular matrix. The levels of mRNA and the amount of protein synthesized are not influenced by cyclic AMP or dexamethasone, factors that have been found to modulate FN expression in other cells. However, the cells are sensitive to the factors, suggesting a cell-specific regulation. The EGF-induced RNA contains the sequences EIIIA and EIIIB characteristic of cellular fibronectin.